LOCUS       sequence-285332-       13192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2640
FEATURES             Location/Qualifiers
     source          1..13192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6119..6128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7156..7173)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    9049..9637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9102..9122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9520..9531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9709..9942
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10020..10141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10057..10076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10111..10130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10181..10316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10336..10355)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10337..10355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10365..10382)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10365..10381)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10374..10396)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10523..10978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10610..10629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10820..10841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11004..11108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11109..11177,11178..11969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11327..11346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12140..12728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12629..12648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12882..12899
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13016..13037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13052..13057,13058..13075,13076..13082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13090..13106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13095..13117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13114..13130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13114..13130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13149..13169
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13151..13169
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtat tcggcccaca ggcccctgat
     4141 gcccgcgtcc agcgcggcct cccgtggcct cggcatgttc gtgtggacga atgtggaacc
     4201 tcgctctgtg gctgtgttcc cttggcactc cttagtcccc ttcctggcac ccagccagcc
     4261 tgacccctcc gtgcagccga gcgaggccca gcaacctgcc agccacccag tggcctccaa
     4321 ccagagcaaa gaacctgctg agtcggcagc tgttgctcat gaacggccac caggtgggac
     4381 agggagtgct gaccctgagc ggccccctgg agccacatgc cctgagagcc caggacccgg
     4441 acccccacac cctttggggg tggtggaatc tggtaagggt ccgcctccca ccacggagga
     4501 ggaggcctcc ggccccccag gagagccccg gctggacagt gagacagaga gtgaccatga
     4561 tgatgccttc ctctccatca tgtctcctga gatccagttg cctctaccgc ccggaaaacg
     4621 tcggacccag tccctcagtg ccctacccaa ggaacgggac tcatcttctg agaaggatgg
     4681 acgcagcccc aacaagcggg agaaggacca catccggcgg cccatgaatg ccttcatgat
     4741 cttcagcaag cggcaccggg ccctggtcca ccagcgtcat cccaaccagg acaaccggac
     4801 cgtcagcaag atcctgggcg agtggtggta tgccctgggg cccaaggaga agcagaagta
     4861 ccacgacctg gccttccagg tgaaggaggc ccacttcaag gcccacccag attggaagtg
     4921 gtgcaacaag gaccgaaaga agtccagctc agaggccaag cccacgagcc tggggctggc
     4981 aggagggcac aaggagacgc gggagcggag catgtcggag acgggcactg ctgctgcccc
     5041 tggggtgtcc tctgagctcc tgtccgttgc agcccagaca ctcctgagct cagacaccaa
     5101 ggctccgggg agcagctcct gtggggcaga acggctacac acagttgggg gacctggctc
     5161 agcccggccc cgagctttct cccacagcgg ggtacacagc ctggacggcg gagaagtaga
     5221 cagtcaggcg ctacaggaac tgacgcagat ggtgtctggc cctgcatcgt actctggccc
     5281 aaagccttct acccagtatg gagctccagg accctttgca gcccctggtg agggaggtgc
     5341 cttggcggcc actgggcggc ccccgctgct gcccacccga gcttctcgtt ctcagcgtgc
     5401 ggccagtgag gacatgacga gtgatgagga gcgcatggtc atctgtgagg aggaagggga
     5461 tgatgatgtc attgctgacg atggcttcgg caccactgac attgatctca agtgcaagga
     5521 gcgggtgacc gacagcgaga gtggggacag ctctggggag gacccagagg gcaacaaggg
     5581 ctttggtcgg aaggtgtttt cacctgtgat ccgttcctcc tttacccact gccgcccccc
     5641 actggaccct gagcccccag ggcccccgga tcctcctgta gcctttggca aaggctatgg
     5701 ttccgcccca tcctcctctg cgtcctcgcc tgcttcctcc tcagcctcgg cagccacctc
     5761 cttctcactg ggctcaggaa ccttcaaggc ccaggagtct ggtcagggca gcacagcggg
     5821 ccccctacgg cccccacccc ctggggctgg gggtccagcg acaccttcca aggcaacccg
     5881 gttcctccca atggatcctg ccaccttccg gcgcaagaga cccgaaagtg tgggtggcct
     5941 ggagccacca ggcccctcag tcatcgcggc ccctcccagc ggaggaggaa acatcctgca
     6001 gacactggtg ctgcccccaa acaaggagga gcaagagggc ggcggagcca gagtgccctc
     6061 cgcccccgcc ccatcactgg cctatggggc cccagcagct cccctgtccc gtcctgccgc
     6121 caccatggtc accaatgtgg tgcggcctgt cagcagcact cctgtgccca tcgcctctaa
     6181 gcccttcccc acctctggcc gggctgaggc gtctccaaat gacacagcag gtgccaggac
     6241 tgaaatgggc actgggtctc gggtgcctgg gggctccccg ctgggtgtca gcttagtgta
     6301 ttcggacaag aagtcggcag cagccacctc accagcccca cacttggtgg ctggacccct
     6361 gctgggcact gtggggaagg cgcctgccac tgtcactaac ctactggtgg gcaccccggg
     6421 gtatggggcc cctgcgcccc ctgctgtcca gttcattgcc cagggggccc ctggtggtgg
     6481 gaccactgcg ggctcaggag caggtgctgg gagtggcccc aatgggccag tacccctggg
     6541 catcctgcaa ccaggtgccc tgggcaaggc tgggggaatc acccaggtac agtacatcct
     6601 gcccacgctg ccccagcagc ttcaggtggc acctgcccca gcaccagccc ctgggaccaa
     6661 ggcagcggct cccagcggcc ctgcacccac caccagcatc cgtttcaccc tcccaccggg
     6721 cacttccacc aacggcaaag tcctggctgc cactgcaccc actcctggca tccccatcct
     6781 gcagtctgta ccctccgccc caccccccaa agcccagtca gtttctcccg tgcaggcccc
     6841 gcccccgggt ggctcagccc agctgctgcc tgggaaggtc ctagtgcctc tggccgcccc
     6901 tagcatgtca gtgcggggtg gaggggccgg ccagccactg ccactggtga gcccgccctt
     6961 ctcagtacct gtgcagaatg gtgcccagcc ccccagcaag atcatccagc tgaccccggt
     7021 gcctgtgagc acacccagcg gcctggtgcc gcccctgagc ccagccacac tccctggacc
     7081 cacctctcag cctcagaagg tcctgttgcc ctcctccacc agaatcacct atgtgcagtc
     7141 agcgggcggg cacgcgctgc ccctgggtac cagccctgcg tccagccagg ctggaacagt
     7201 cacctcgtac gggcccacga gctctgtagc tctaggcttc acctcgctgg ggcccagcgg
     7261 ccccgccttc gtgcagcccc tgctctcagc aggccaagcc ccactgctgg ctcccggtca
     7321 ggtgggcgtg tcacctgtgc ccagtcccca gctgccgcct gcctgtgcag cccccggagg
     7381 tcctgtcata acagcatttt actctggcag ccctgcaccc acctcctcag cacccctggc
     7441 ccagccatcc caggcccccc caagcctggt ctacactgtg gccaccagca caaccccacc
     7501 tgcagccacc attctgccca agggcccgcc agcccctgcc actgccaccc cagccccgac
     7561 tagccctttc cccagcgcca cagcaggttc catgacctac agcttagtgg cccccaaggc
     7621 ccagcggccc agcccgaagg ccccccagaa agtgaaggca gccatcgcca gcattcccgt
     7681 ggggtccttt gaggcaggtg cctctgggcg gcctggccct gcaccccggc agcctctgga
     7741 gcctggccca gtccgagagc caactgcccc agagtctgag cttgaggggc agcccacacc
     7801 accagcccct ccacccctgc cagagacctg gactcccacg gcccggagca gccccccact
     7861 gcccccacct gctgaggagc ggaccagcgc caagggccct gagaccatgg ccagcaaatt
     7921 ccccagctca tcttcagact ggcgcgtccc tgggcagggc ctggagaatc gtggggagcc
     7981 tcccactcct cccagcccgg ccccagctcc agctgtagcc cctggtggca gcagcgagag
     8041 cagcagtggg cgggcagccg gggacacccc ggagcgcaag gaggcggctg gtactggcaa
     8101 gaaggtgaag gtgcggcccc cgcccctgaa gaagaccttt gactctgtgg acaacagggt
     8161 cctgtcagaa gtggacttcg aagagcgctt tgctgagttg cctgagtttc ggcctgagga
     8221 ggtgctgccc tcccccaccc tgcagtctct ggccacctca ccccgggcca tcctgggctc
     8281 ttaccgcaag aagaggaaga actccacgga cctggattca gcacccgagg accccacctc
     8341 gcccaagcgc aagatgagaa gacgctccag ctgcagctcg gagcccaaca cccccaagag
     8401 tgccaagtgc gagggggaca tcttcacctt tgaccgtaca ggtacagaag ccgaggacgt
     8461 gcttggggag ctagagtatg acaaggtgcc atactcctcc ctgcggcgca ccctggacca
     8521 gcgccgggcc ctggtcatgc agctctttca ggaccatggc ttcttcccgt cagcccaggc
     8581 cacagccgcc ttccaggccc gctatgcaga catctttccc tccaaggttt gtctgcagtt
     8641 gaagatccgt gaggtgcgcc agaagatcat gcaggctgcc actcccacgg agcagccccc
     8701 tggagctgag gctcctctcc ctgtaccgcc ccccactggc accgctgctg cccctgcccc
     8761 cactcccagc cccgcagggg gccctgaccc cacctcaccc agctcggact ctggcacggc
     8821 ccaggctgcc ccgccactgc ctccaccccc agagtcgggg cctggacagc ctggctggga
     8881 gggggctccc cagccctccc ccccaccccc aggtccctcc acagctgcca caggcaggta
     8941 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     9001 aaaggacgac aaccgccaaa ggaccgctgc gacacgcgtt aagtcgacaa tcaacctctg
     9061 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     9121 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     9181 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     9241 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     9301 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     9361 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     9421 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     9481 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9541 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9601 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     9661 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     9721 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     9781 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9841 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9901 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     9961 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
    10021 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10081 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10141 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
    10201 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    10261 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10321 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
    10381 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    10441 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    10501 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    10561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    10621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    10681 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    10741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
    10801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10861 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    10921 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    10981 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11041 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11101 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11161 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11221 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11281 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11341 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11401 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11461 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    11521 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    11581 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    11641 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    11701 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    11761 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    11821 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11881 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11941 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12001 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12061 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12361 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12421 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12481 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    12541 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    12601 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    12661 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    12721 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    12781 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12841 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12901 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12961 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    13021 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    13081 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    13141 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
