LOCUS       sequence-285336-        1376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2931
FEATURES             Location/Qualifiers
     source          1..1376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1208..1232)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1240..1281
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgcagagtgc gactgtcccc
       61 gcggaagggg ctgtcaaggg gctcccggag atgctaggtg tgccgatgca acagattccc
      121 cagtgcgctg gctgcaacca gcacatcctg gacaagttca tcctgaaggt cctggacaga
      181 cactggcaca gctcctgcct caagtgtgca gactgccaga tgcagctggc ggacaggtgc
      241 ttctccaggg ctgggagcgt ctactgcaag gaggacttct tcaagcgctt cggcacaaaa
      301 tgcacggcct gccagcaggg tatcccccca acccaggtgg tccgcaaggc ccaggacttt
      361 gtctaccacc tgcactgctt tgcttgcatc atctgcaacc ggcagctggc cacgggggac
      421 gaattctacc tcatggagga cgggcggctg gtgtgcaagg aagactacga gacagccaag
      481 cagaacgatg actcagaggc tggagctaag cggccccgga ccaccatcac agccaagcag
      541 ctggagacat taaagaatgc atacaagaac tcccccaagc ctgcccggca cgtgagggag
      601 cagctgtcct cagagacagg cctggacatg agggtcgtac aggtttggtt tcagaacaga
      661 agggccaaag agaaacgcct gaagaaggat gcagggcggc accgctgggg gcagttctat
      721 aagagcgtca agaggagccg gggcagcagc aagcaggaga aggagagctc tgcagaggac
      781 tgtggggtta gtgacagtga gctgagcttc cgagaggatc aaattctctc agaacttggc
      841 cacaccaata ggatttatgg caacgtgggg gacgttacag gcggacagtt aatgaatggg
      901 agcttctcca tggacgggac aggacaatcc tatcaggact tgagggatgg gagcccctat
      961 ggaatccccc agtctccatc ctccatatcg tccctgccat cccacgctcc tttgctcaat
     1021 gggctggatt acacggtgga cagtaatttg ggcatcattg cgcatgcagg gcagggagta
     1081 agccagacgc tgagagccat ggctggggga cccacctctg acatctccac aggaagcagt
     1141 gtaggctatc ccgactttcc aactagccca ggctcttggc tcgatgaaat ggatcatcct
     1201 cctttttacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1261 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1321 tcttggcttt atatatcttg tggaaaggac gacccaagct attcgcccat ctaacg
//
