LOCUS       sequence-285339-        1499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2932
FEATURES             Location/Qualifiers
     source          1..1499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1331..1355)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1363..1404
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tagttcacag tttttcagcc
       61 atggaccgtc acgacggcac cagcaacggg acggcacggt tgccccagct gggcactgta
      121 ggtcaatctc cctacacgag cgccccgccg ctgtcccaca cccccaatgc cgacttccag
      181 cccccatact tccccccacc ctaccagcct atctaccccc agtcgcaaga tccttactcc
      241 cacgtcaacg acccctacag cctgaacccc ctgcacgccc agccgcagcc gcagcaccca
      301 ggctggcccg gccagaggca gagccaggag tctgggctcc tgcacacgca ccgggggctg
      361 cctcaccagc tgtcgggcct ggatcctcgc agggactaca ggcggcacga ggacctcctg
      421 cacggcccac acgcgctcag ctcaggactc ggagacctct cgatccactc cttacctcac
      481 gccatcgagg aggtcccgca tgtagaagac ccgggtatta acatcccaga tcaaactgta
      541 attaagaaag gccccgtgtc cctgtccaag tccaacagca atgccgtctc cgccatccct
      601 attaacaagg acaacctctt cggcggcgtg gtgaacccca acgaagtctt ctgttcagtt
      661 ccgggtcgcc tctcgctcct cagctccacc tcgaagtaca aggtcacggt ggcggaagtg
      721 cagcggcggc tctcaccacc cgagtgtctc aacgcgtcgc tgctgggcgg agtgctccgg
      781 agggcgaagt ctaaaaatgg aggaagatct ttaagagaaa aactggacaa aataggatta
      841 aatctgcctg cagggagacg taaagctgcc aacgttaccc tgctcacatc actagtagag
      901 ggagaagctg tccacctagc cagggacttt gggtacgtgt gcgaaaccga atttcctgcc
      961 aaagcagtag ctgaatttct caaccgacaa cattccgatc ccaatgagca agtgacaaga
     1021 aaaaacatgc tcctggctac aaaacagata tgcaaagagt tcaccgacct gctggctcag
     1081 gaccgatctc ccctggggaa ctcacggccc aaccccatcc tggagcccgg catccagagc
     1141 tgcttgaccc acttcaacct catctcccac ggcttcggca gccccgcggt gtgtgccgcg
     1201 gtcacggccc tgcagaacta tctcaccgag gccctcaagg ccatggacaa aatgtacctc
     1261 agcaacaacc ccaatagcca cacggacaac aacgccaaaa gcagtgacaa agaggagaag
     1321 cacagaaagt gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1381 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1441 atttcttggc tttatatatc ttgtggaaag gacgagagga aggtatgtct caagagcct
//
