LOCUS       sequence-285345-        1634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2934
FEATURES             Location/Qualifiers
     source          1..1634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1466..1490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1498..1539
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgtcacgcat agggttgcgc
       61 atgcagctca tgcgggagca ggcgcagcag gaggagcagc gggagcgcat gcagcaacag
      121 gctgtcatgc attacatgca gcagcagcag cagcagcaac agcagcagct cggagggccg
      181 cccaccccgg ccatcaatac ccccgtccac ttccagtcgc caccacctgt gcctggggag
      241 gtgttgaagg tgcagtccta cctggagaat cccacatcct accatctgca gcagtcgcag
      301 catcagaagg tgcgggagta cctgtccgag acctatggga acaagtttgc tgcccacatc
      361 agcccagccc agggctctcc gaaaccccca ccagccgcct ccccaggggt gcgagctgga
      421 cacgtgctgt cctcctccgc tggcaacagt gctcccaata gccccatggc catgctgcac
      481 attggctcca accctgagag ggagttggat gatgtcattg acaacattat gcgtctggac
      541 gatgtccttg gctacatcaa tcctgaaatg cagatgccca acacgctacc cctgtccagc
      601 agccacctga atgtgtacag cagcgacccc caggtcacag cctccctggt gggcgtcacc
      661 agcagctcct gccctgcgga cctgacccag aagcgagagc tcacagatgc tgagagcagg
      721 gccctggcca aggagcggca gaagaaagac aatcacaact taattgaaag gagacgaagg
      781 ttcaacatca atgaccgcat caaggagttg ggaatgctga tccccaaggc caatgacctg
      841 gacgtgcgct ggaacaaggg caccatcctc aaggcctctg tggattacat ccggaggatg
      901 cagaaggacc tgcaaaagtc cagggagctg gagaaccact ctcgccgcct ggagatgacc
      961 aacaagcagc tctggctccg tatccaggag ctggagatgc aggctcgagt gcacggcctc
     1021 cctaccacct ccccgtccgg catgaacatg gctgagctgg cccagcaggt ggtgaagcag
     1081 gagctgccta gcgaagaggg cccaggggag gccctgatgc tgggggctga ggtccctgac
     1141 cctgagccac tgccagctct gcccccgcaa gccccgctgc ccctgcccac ccagccacca
     1201 tccccattcc atcacctgga cttcagccac agcctgagct ttgggggcag ggaggacgag
     1261 ggtcccccgg gctaccccga acccctggcg ccggggcatg gctccccatt ccccagcctg
     1321 tccaagaagg atctggacct catgctcctg gacgactcac tgctaccgct ggcctctgat
     1381 ccacttctgt ccaccatgtc ccccgaggcc tccaaggcca gcagccgccg gagcagcttc
     1441 agcatggagg agggcgatgt gctgtgccca actttcttgt acaaagtggt tgatatcggt
     1501 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1561 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tgcgcacgat
     1621 attattttta cctt
//
