LOCUS       sequence-285351-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2936
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1178..1202)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1210..1251
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgttctcggg cttcaacgca
       61 gactacgagg cgtcatcctc ccgctgcagc agcgcgtccc cggccgggga tagcctctct
      121 tactaccact cacccgcaga ctccttctcc agcatgggct cgcctgtcaa cgcgcaggac
      181 ttctgcacgg acctggccgt ctccagtgcc aacttcattc ccacggtcac tgccatctcg
      241 accagtccgg acctgcagtg gctggtgcag cccgccctcg tctcctctgt ggccccatcg
      301 cagaccagag cccctcaccc tttcggagtc cccgccccct ccgctggggc ttactccagg
      361 gctggcgttg tgaagaccat gacaggaggc cgagcgcaga gcattggcag gaggggcaag
      421 gtggaacagt tatctccaga agaagaagag aaaaggagaa tccgaaggga aaggaataag
      481 atggctgcag ccaaatgccg caaccggagg agggagctga ctgatacact ccaagcggag
      541 acagaccaac tagaagatga gaagtctgct ttgcagaccg agattgccaa cctgctgaag
      601 gagaaggaaa aactagagtt catcctggca gctcaccgac ctgcctgcaa gatccctgat
      661 gacctgggct tcccagaaga gatgtctgtg gcttcccttg atctgactgg gggcctgcca
      721 gaggttgcca ccccggagtc tgaggaggcc ttcaccctgc ctctcctcaa tgaccctgag
      781 cccaagccct cagtggaacc tgtcaagagc atcagcagca tggagctgaa gaccgagccc
      841 tttgatgact tcctgttccc agcatcatcc aggcccagtg gctctgagac agcccgctcc
      901 gtgccagaca tggacctatc tgggtccttc tatgcagcag actgggagcc tctgcacagt
      961 ggctccctgg ggatggggcc catggccaca gagctggagc ccctgtgcac tccggtggtc
     1021 acctgtactc ccagctgcac tgcttacacg tcttccttcg tcttcaccta ccccgaggct
     1081 gactccttcc ccagctgtgc agctgcccac cgcaagggca gcagcagcaa tgagccttcc
     1141 tctgactcgc tcagctcacc cacgctgctg gccctgtgcc caactttctt gtacaaagtg
     1201 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1261 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1321 gaattgtcaa tggtttgatc tcagca
//
