LOCUS       sequence-285357-        2132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2938
FEATURES             Location/Qualifiers
     source          1..2132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1964..1988)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1996..2037
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cttccaagcg accagcctct
       61 ccgtatgggg aagcagatgg agaggtagcc atggtgacaa gcagacagaa agtggaagaa
      121 gaggagagtg acgggctccc agcctttcac cttcccttgc atgtgagttt tcccaacaag
      181 cctcactctg aggaatttca gccagtttct ctgctgacgc aagagacttg tggccatagg
      241 actcccactt ctcagcacaa tacaatggaa gttgatggca ataaagttat gtcttcattt
      301 gccccacaca actcatctac ctcacctcag aaggcagaag aaggtgggcg acagagtggc
      361 gagtccttgt ctagtacagc cctgggaact cctgaacggc gcaagggcag tttagctgat
      421 gttgttgaca ccttgaagca gaggaaaatg gaagagctca tcaaaaacga gccggaagaa
      481 acccccagta ttgaaaaact actctcaaag gactggaaag acaagcttct tgcaatggga
      541 tcggggaact ttggcgaaat aaaagggact cccgagagct tagctgagaa agaaaggcaa
      601 ctcatgggta tgatcaacca gctgaccagc ctccgagagc agctgttggc tgcccacgat
      661 gagcagaaga aactagctgc ctctcagatt gagaaacagc gtcagcaaat ggagctggcc
      721 aagcagcaac aagaacaaat tgcaagacag cagcagcagc ttctacagca acaacacaaa
      781 atcaatttgc tccagcaaca gatccaggtt caaggtcagc tgccgccatt aatgattccc
      841 gtattccctc ctgatcaacg gacactggct gcagctgccc agcaaggatt cctcctccct
      901 ccaggcttca gctataaggc tggatgtagt gacccttacc ctgttcagct gatcccaact
      961 accatggcag ctgctgccgc agcaacacca ggcttaggcc cactccaact gcagcagtta
     1021 tatgctgccc agctagctgc aatgcaggta tctccaggag ggaagctgcc aggcataccc
     1081 caaggcaacc ttggtgctgc tgtatctcct accagcattc acacagacaa gagcacaaac
     1141 agcccaccac ccaaaagcaa ggaaaaaaca acactggaga gtctgactca gcaactggca
     1201 gttaaacaga atgaagaagg aaaatttagc catgcaatga tggatttcaa tctgagtgga
     1261 gattctgatg gaagtgctgg agtctcagag tcaagaattt atagggaatc ccgagggcgt
     1321 ggtagcaatg aaccccacat aaagcgtcca atgaatgcct tcatggtgtg ggctaaagat
     1381 gaacggagaa agatccttca agcctttcct gacatgcaca actccaacat cagcaagata
     1441 ttgggatctc gctggaaagc tatgacaaac ctagagaaac agccatatta tgaggagcaa
     1501 gcccgtctca gcaagcagca cctggagaag taccctgact ataagtacaa gcccaggcca
     1561 aagcgcacct gcctggtgga tggcaaaaag ctgcgcattg gtgaatacaa ggcaatcatg
     1621 cgcaacaggc ggcaggaaat gcggcagtac ttcaatgttg ggcaacaagc acagatcccc
     1681 attgccactg ctggtgttgt gtaccctgga gccatcgcca tggctgggat gccctcccct
     1741 cacctgccct cggagcactc aagcgtgtct agcagcccag agcctgggat gcctgttatc
     1801 cagagcactt acggtgtgaa aggagaggag ccacatatca aagaagagat acaggccgag
     1861 gacatcaatg gagaaattta tgatgagtac gacgaggaag aggatgatcc agatgtagat
     1921 tatgggagtg acagtgaaaa ccatattgca ggacaagcca actgcccaac tttcttgtac
     1981 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     2041 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2101 aaggacgata gagctaccag gtatcccgcg cg
//
