LOCUS       sequence-285360-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2939
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(737..761)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             769..810
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gttcctattt tgtgaatccc
       61 actttccccg ggagccttcc cagcggccag gactccttct tgggccagct gcccctctac
      121 caggctggct atgacgcgct gaggcccttc ccggcctcgt acggggcgtc gagtctcccg
      181 gacaagacgt acacctcacc ttgtttctac caacagtcca actcggtcct ggcctgcaac
      241 cgggcgtcct acgagtacgg ggcctcgtgt ttctattctg ataaggacct cagtggcgcc
      301 tcgccctcgg gcagtggcaa gcagaggggc cccggggact acctgcactt ttctcccgag
      361 cagcagtaca aacccgacag cagcagcggg cagggcaaag cactccatga cgaaggcgcc
      421 gaccggaagt acacgagccc ggtttaccct tggatgcagc ggatgaactc ctgcgcgggt
      481 gctgtgtatg ggagccatgg gcgccgaggc cgccagacct acacgcgcta ccagacactg
      541 gagctggaga aggagttcca cttcaaccgc tacctgacac ggcgccgccg catcgagatc
      601 gccaacgcgc tctgcctcac cgagcgccag atcaagatct ggttccagaa ccgccgcatg
      661 aagtggaaaa aggaaaacaa gctcatcaat tccacgcagc ccagcgggga ggactcagag
      721 gcaaaggcgg gcgagtaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      781 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      841 atttcgattt cttggcttta tatatcttgt ggaaaggacg aatacacata aggattattc
      901 ccccg
//
