LOCUS       sequence-285363-        2000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2940
FEATURES             Location/Qualifiers
     source          1..2000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1832..1856)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1864..1905
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cctgtatcca agcccaatat
       61 gggacaccag caccgagtcc gggaccccgt gaccacctgg caagcgaccc cctgacccct
      121 gagttcatca agcccaccat ggacctggcc agccccgagg cagcccccgc tgcccccact
      181 gccctgccca gcttcagcac cttcatggac ggctacacag gagagtttga caccttcctc
      241 taccagctgc caggaacagt ccagccatgc tcctcagcct cctcctcggc ctcctccaca
      301 tcctcgtcct cagccacctc ccctgcctct gcctccttca agttcgagga cttccaggtg
      361 tacggctgct accccggccc cctgagcggc ccagtggatg aggccctgtc ctccagtggc
      421 tctgactact atggcagccc ctgctcggcc ccgtcgccct ccacgcccag cttccagccg
      481 ccccagctct ctccctggga tggctccttc ggccacttct cgcccagcca gacttacgaa
      541 ggcctgcggg catggacaga gcagctgccc aaagcctctg ggcccccaca gcctccagcc
      601 ttcttttcct tcagtcctcc caccggcccc agccccagcc tggcccagag ccccctgaag
      661 ttgttcccct cacaggccac ccaccagctg ggggagggag agagctattc catgcctacg
      721 gccttcccag gtttggcacc cacttctcca caccttgagg gctcggggat actggataca
      781 cccgtgacct caaccaaggc ccggagcggg gccccaggtg gaagtgaagg ccgctgtgct
      841 gtgtgtgggg acaacgcttc atgccagcat tatggtgtcc gcacatgtga gggctgcaag
      901 ggcttcttca agcgcacagt gcagaaaaac gccaagtaca tctgcctggc taacaaggac
      961 tgccctgtgg acaagaggcg gcgaaaccgc tgccagttct gccgcttcca gaagtgcctg
     1021 gcggtgggca tggtgaagga agttgtccga acagacagcc tgaaggggcg gcggggccgg
     1081 ctaccttcaa aacccaagca gcccccagat gcctcccctg ccaatctcct cacttccctg
     1141 gtccgtgcac acctggactc agggcccagc actgccaaac tggactactc caagttccag
     1201 gagctggtgc tgccccactt tgggaaggaa gatgctgggg atgtacagca gttctacgac
     1261 ctgctctccg gttctctgga ggtcatccgc aagtgggcgg agaagatccc tggctttgct
     1321 gagctgtcac cggctgacca ggacctgttg ctggagtcgg ccttcctgga gctcttcatc
     1381 ctccgcctgg cgtacaggtc taagccaggc gagggcaagc tcatcttctg ctcaggcctg
     1441 gtgctacacc ggctgcagtg tgcccgtggc ttcggggact ggattgacag tatcctggcc
     1501 ttctcaaggt ccctgcacag cttgcttgtc gatgtccctg ccttcgcctg cctctctgcc
     1561 cttgtcctca tcaccgaccg gcatgggctg caggagccgc ggcgggtgga ggagctgcag
     1621 aaccgcatcg ccagctgcct gaaggagcac gtggcagctg tggcgggcga gccccagcca
     1681 gccagctgcc tgtcacgtct gttgggcaaa ctgcccgagc tgcggaccct gtgcacccag
     1741 ggcctgcagc gcatcttcta cctcaagctg gaggacttgg tgccccctcc acccatcatt
     1801 gacaagatct tcatggacac gctgcccttc tgcccaactt tcttgtacaa agtggttgat
     1861 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1921 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatgac
     1981 ccaggtaaca taaacgcctc
//
