LOCUS       sequence-285366-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2941
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(905..929)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             937..978
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga caggagtgtt tgacagaagg
       61 gtccccagca tccgatccgg cgacttccaa gctccgttcc agacgtccgc agctatgcac
      121 catccgtctc aggaatcgcc aactttgccc gagtcttcag ctaccgattc tgactactac
      181 agccctacgg ggggagcccc gcacggctac tgctctccta cctcggcttc ctatggcaaa
      241 gctctcaacc cctaccagta tcagtatcac ggcgtgaacg gctccgccgg gagctaccca
      301 gccaaagctt atgccgacta tagctacgct agctcctacc accagtacgg cggcgcctac
      361 aaccgcgtcc caagcgccac caaccagcca gagaaagaag tgaccgagcc cgaggtgaga
      421 atggtgaatg gcaaaccaaa gaaagttcgt aaacccagga ctatttattc cagctttcag
      481 ctggccgcat tacagagaag gtttcagaag actcagtacc tcgccttgcc ggaacgcgcc
      541 gagctggccg cctcgctggg attgacacaa acacaggtga aaatctggtt tcagaacaaa
      601 agatccaaga tcaagaagat catgaaaaac ggggagatgc ccccggagca cagtcccagc
      661 tccagcgacc caatggcgtg taactcgccg cagtctccag cggtgtggga gccccagggc
      721 tcgtcccgct cgctcagcca ccaccctcat gcccaccctc cgacctccaa ccagtcccca
      781 gcgtccagct acctggagaa ctctgcatcc tggtacacaa gtgcagccag ctcaatcaat
      841 tcccacctgc cgccgccggg ctccttacag cacccgctgg cgctggcctc cgggacactc
      901 tattacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
      961 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1021 tggctttata tatcttgtgg aaaggacgat taatgtacca gcgcatcaat ggc
//
