LOCUS       sequence-285369-        1532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2942
FEATURES             Location/Qualifiers
     source          1..1532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             162..173
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1364..1388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1396..1437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt caaacaaaga tcgacacatt
       61 gattccagct gttcgtcctt catcaagacg gaaccttcca gcccagcctc cctgacggac
      121 agcgtcaacc accacagccc tggtggctct tcagacgcca gtgggagcta cagttcaacc
      181 atgaatggcc atcagaacgg acttgactcg ccacctctct acccttctgc tcctatcctg
      241 ggaggtagtg ggcctgtcag gaaactgtat gatgactgct ccagcaccat tgttgaagat
      301 ccccagacca agtgtgaata catgctcaac tcgatgccca agagactgtg tttagtgtgt
      361 ggtgacatcg cttctgggta ccactatggg gtagcatcat gtgaagcctg caaggcattc
      421 ttcaagagga caattcaagg caatatagaa tacagctgcc ctgccacgaa tgaatgtgaa
      481 atcacaaagc gcagacgtaa atcctgccag gcttgccgct tcatgaagtg tttaaaagtg
      541 ggcatgctga aagaaggggt gcgtcttgac agagtacgtg gaggtcggca gaagtacaag
      601 cgcaggatag atgcggagaa cagcccatac ctgaaccctc agctggttca gccagccaaa
      661 aagccattgc tctggtctga tcctgcagat aacaagattg tctcacattt gttggtggct
      721 gaaccggaga agatctatgc catgcctgac cctactgtcc ccgacagtga catcaaagcc
      781 ctcactacac tgtgtgactt ggccgaccga gagttggtgg ttatcattgg atgggcgaag
      841 catattccag gcttctccac gctgtccctg gcggaccaga tgagccttct gcagagtgct
      901 tggatggaaa ttttgatcct tggtgtcgta taccggtctc tttcgtttga ggatgaactt
      961 gtctatgcag acgattatat aatggacgaa gaccagtcca aattagcagg ccttcttgat
     1021 ctaaataatg ctatcctgca gctggtaaag aaatacaaga gcatgaagct ggaaaaagaa
     1081 gaatttgtca ccctcaaagc tatagctctt gctaattcag actccatgca catagaagat
     1141 gttgaagccg ttcagaagct tcaggatgtc ttacatgaag cgctgcagga ttatgaagct
     1201 ggccagcaca tggaagaccc tcgtcgagct ggcaagatgc tgatgacact gccactcctg
     1261 aggcagacct ctaccaaggc cgtgcagcat ttctacaaca tcaaactaga aggcaaagtc
     1321 ccaatgcaca aacttttttt ggaaatgttg gaggccaagg tctgcccaac tttcttgtac
     1381 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1441 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1501 aaggacgagt ctcaaagcgc gttagtgagg aa
//
