LOCUS       sequence-285372-        1520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2943
FEATURES             Location/Qualifiers
     source          1..1520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             168..179
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(1352..1376)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1384..1425
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatgtcaaa caaagatcga
       61 cacattgatt ccagctgttc gtccttcatc aagacggaac cttccagccc agcctccctg
      121 acggacagcg tcaaccacca cagccctggt ggctcttcag acgccagtgg gagctacagt
      181 tcaaccatga atggccatca gaacggactt gactcgccac ctctctaccc ttctgctcct
      241 atcctgggag gtagtgggcc tgtcaggaaa ctgtatgatg actgctccag caccattgtt
      301 gaagatcccc agaccaagtg tgaatacatg ctcaactcga tgcccaagag actgtgttta
      361 gtgtgtggtg acatcgcttc tgggtaccac tatggggtag catcatgtga agcctgcaag
      421 gcattcttca agaggacaat tcaaggcaat atagaataca gctgccctgc cacgaatgaa
      481 tgtgaaatca caaagcgcag acgtaaatcc tgccaggctt gccgcttcat gaagtgttta
      541 aaagtgggca tgctgaaaga aggggtgcgt cttgacagag tacgtggagg tcggcagaag
      601 tacaagcgca ggatagatgc ggagaacagc ccatacctga accctcagct ggttcagcca
      661 gccaaaaagc catataacaa gattgtctca catttgttgg tggctgaacc ggagaagatc
      721 tatgccatgc ctgaccctac tgtccccgac agtgacatca aagccctcac tacactgtgt
      781 gacttggccg accgagagtt ggtggttatc attggatggg cgaagcatat tccaggcttc
      841 tccacgctgt ccctggcgga ccagatgagc cttctgcaga gtgcttggat ggaaattttg
      901 atccttggtg tcgtataccg gtctctttca tttgaggatg aacttgtcta tgcagacgat
      961 tatataatgg acgaagacca gtccaaatta gcaggccttc ttgatctaaa taatgctatc
     1021 ctgcagctgg taaagaaata caagagcatg aagctggaaa aagaagaatt tgtcaccctc
     1081 aaagctatag ctcttgctaa ttcagactcc atgcacatag aagatgttga agccgttcag
     1141 aagcttcagg atgtcttaca tgaagcgctg caggattatg aagctggcca gcacatggaa
     1201 gaccctcgtc gagctggcaa gatgctgatg acactgccac tcctgaggca gacctctacc
     1261 aaggccgtgc agcatttcta caacatcaaa ctagaaggca aagtcccaat gcacaaactt
     1321 tttttggaaa tgttggaggc caaggtctga gacccagctt tcttgtacaa agtggttgat
     1381 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1441 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaacta
     1501 gtaatctgca acaaaaacct
//
