LOCUS       sequence-285375-        2408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2944
FEATURES             Location/Qualifiers
     source          1..2408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2240..2264)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2272..2313
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagcggcg gagcgagagc
       61 ccgtgtctgc gggacagccc cgaccggcgg agcggcagcc cggacgtcaa ggggcctccc
      121 ccagtgaagg tggcccggct ggagcagaac ggcagcccca tgggagcccg cgggaggccc
      181 aacggcgccg tggccaaggc cgtgggaggt ttgatgattc ctgtcttttg tgtcgtggag
      241 cagttggacg gctctcttga atatgacaac agagaagaac acgccgagtt tgtcctggtg
      301 cggaaagatg tgctttttag ccagctggtg gagactgcgc tcctggccct ggggtattct
      361 cacagctctg cggcccaggc ccaaggaata atcaagctgg gaaggtggaa ccctctcccc
      421 ctcagttatg tgacagatgc acccgacgcg acagtggccg acatgctaca agatgtctat
      481 catgttgtga cgttgaaaat ccaattacaa agttgttcaa agttggaaga cttgcctgcg
      541 gagcagtgga accatgccac agtccgcaat gccttaaagg aactgctcaa agagatgaac
      601 cagagcacat tagccaaaga atgccctctc tcccagagta tgatttcatc cattgtaaat
      661 agcacatatt atgccaatgt gtcagcaacc aagtgccagg agtttgggag atggtataaa
      721 aagtacaaga agattaaagt ggaaagagtg gaacgagaaa acctttcaga ctattgtgtt
      781 ctgggccagc gtccaatgca tttaccaaat atgaaccagc tggcatccct ggggaaaacc
      841 aacgaacagt ctcctcacag ccaaattcac cacagtactc caatccgaaa ccaagtgccc
      901 gcattacagc ccatcatgag ccctggtctt ctttctcccc agcttagtcc acaacttgta
      961 aggcaacaaa tagccatggc ccatctgata aaccaacaga ttgccgttag ccggctcctg
     1021 gctcaccagc atcctcaagc catcaaccag cagttcctga accatccacc catccccaga
     1081 gcagttaagc cagagccaac caactcttcc gtggaagtct ctccagatat ctaccagcaa
     1141 gtcagagatg agctgaagag ggccagtgtg tcccaagctg tctttgcaag agtggcattc
     1201 aaccgcacac agggattgtt gtctgagatt ctgcgtaagg aagaagaccc tcggacagcc
     1261 tctcagtctc ttctagtaaa cctgagggcc atgcagaatt tcctcaatct gccagaagtg
     1321 gagcgagatc gcatctacca ggatgagagg gagcggagca tgaatcccaa tgtgagcatg
     1381 gtctcctcgg cctccagcag tcccagctcc tcccgaaccc ctcaggccaa aacctcgaca
     1441 ccgacaacag acctccctat taaggtggac ggcgccaaca tcaacatcac agctgccatt
     1501 tatgacgaga tccaacagga gatgaaaagg gccaaggtgt ctcaagccct gtttgccaaa
     1561 gtggctgcaa ataaaagtca gggctggctg tgtgaactgc tccgctggaa ggagaaccca
     1621 agcccagaaa accgcaccct ctgggaaaac ctctgtacca tccgtcgctt cctgaacctt
     1681 ccccagcatg agagggatgt catctatgag gaggagtcaa ggcatcacca cagcgaacgc
     1741 atgcaacacg tggtccagct tccccctgag ccggtgcagg tacttcatag acagcagtct
     1801 cagccagcca aggagagttc ccctcccaga gaagaagcgc ctcccccacc tcctccgact
     1861 gaagacagtt gtgccaaaaa gccccggtct cgcacaaaga tctccttaga agccctgggg
     1921 atcctccaaa gctttattca tgatgtaggc ctgtacccag accaggaagc catccacact
     1981 ctttcggctc agctggatct ccccaaacac accatcatca agttcttcca gaaccagcgg
     2041 taccacgtga agcaccacgg gaagctgaaa gagcacctgg gctccgcggt ggacgtggct
     2101 gaatataagg acgaggagct gctgaccgag tcagaggaga acgacagcga ggaaggctcc
     2161 gaggagatgt acaaagtgga ggctgaggag gaaaatgctg acaaaagcaa ggcagcacct
     2221 gccgaaattg accagagatt gccaactttc ttgtacaaag tggttgatat cggtaagcct
     2281 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     2341 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagtgctt ggtgtgtata
     2401 ttggtgta
//
