LOCUS       sequence-285381-        1166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2946
FEATURES             Location/Qualifiers
     source          1..1166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(998..1022)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1030..1071
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agctactgtc gccaccgctc
       61 cgcgacgtag acctgacggc ccccgacggc tctctctgct cctttgccac aacggacgac
      121 ttctatgacg acccgtgttt cgactccccg gacctgcgct tcttcgaaga cctggacccg
      181 cgcctgatgc acgtgggcgc gctcctgaaa cccgaagagc actcgcactt ccccgcggcg
      241 gtgcacccgg ccccgggcgc acgtgaggac gagcatgtgc gcgcgcccag cgggcaccac
      301 caggcgggcc gctgcctact gtgggcctgc aaggcgtgca agcgcaagac caccaacgcc
      361 gaccgccgca aggccgccac catgcgcgag cggcgccgcc tgagcaaagt aaatgaggcc
      421 tttgagacac tcaagcgctg cacgtcgagc aatccaaacc agcggttgcc caaggtggag
      481 atcctgcgca acgccatccg ctatatcgag ggcctgcagg ctctgctgcg cgaccaggac
      541 gccgcgcccc ctggcgccgc agccgccttc tatgcgccgg gcccgctgcc cccgggccgc
      601 ggcggcgagc actacagcgg cgactccgac gcgtccagcc cgcgctccaa ctgctccgac
      661 ggcatgatgg actacagcgg ccccccgagc ggcgcccggc ggcggaactg ctacgaaggc
      721 gcctactaca acgaggcgcc cagcgaaccc aggcccggga agagtgcggc ggtgtcgagc
      781 ctagactgcc tgtccagcat cgtggagcgc atctccaccg agagccctgc ggcgcccgcc
      841 ctcctgctgg cggacgtgcc ttctgagtcg cctccgcgca ggcaagaggc tgccgccccc
      901 agcgagggag agagcagcgg cgaccccacc cagtcaccgg acgccgcccc gcagtgccct
      961 gcgggtgcga accccaaccc gatataccag gtgctctgcc caactttctt gtacaaagtg
     1021 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1081 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1141 gacgcccgac tctaatcaag gcaggg
//
