LOCUS       sequence-285384-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2947
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(764..788)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             796..837
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gctcctactt cgtcaacccc
       61 ctgttctcca aatacaaagc cggcgagtcc ctggaaccgg cctattacga ctgccggttc
      121 cctcagagcg tgggcaggag ccatgcgctg gtgtacgggc ccggcggctc ggcgcccggc
      181 ttccagcacg cttcgcacca cgttcaagac ttcttccacc acggcacctc cggcatctcc
      241 aactcaggct accagcagaa cccgtgctcg cttagctgcc acggagacgc ctccaaattc
      301 tatggctacg aggcgctccc cagacagtcc ctttatgggg ctcagcaaga ggcgagcgtg
      361 gtgcaatatc ccgactgtaa atcctccgcc aacactaaca gtagcgaagg acaaggccac
      421 ttaaatcaaa actcgtctcc cagcctcatg tttccatgga tgagacccca cgctccgggg
      481 aggcgcagtg gacggcaaac ttacagccgg tatcagacct tggaactaga aaaggagttt
      541 ctctttaatc cttatttgac acgaaaacgt cggattgaag tctctcatgc cctgggactg
      601 accgagagac aagtgaagat ctggttccag aaccgaagga tgaagtggaa aaaggagaac
      661 aacaaggata aactgccggg agcccgagat gaggagaagg tggaggaaga aggaaatgag
      721 gaagaggaga aagaagagga ggaaaaggaa gaaaacaagg actacccaac tttcttgtac
      781 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
      841 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      901 aaggacgagg accgggttaa ggaattttgt tt
//
