LOCUS       sequence-285387-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2948
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(710..734)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             742..783
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gttcctattt cgtgaactcc
       61 accttccccg tcactctggc cagcgggcag gagtccttcc tgggccagct accgctctat
      121 tcgtcgggct atgcggaccc gctgagacat taccccgcgc cctacgggcc agggccgggc
      181 caggacaagg gctttgccac ttcctcctat tacccgccgg cgggcggtgg ctacggccga
      241 gcggcgccct gcgactacgg gccggcgccg gccttctacc gcgagaaaga gtcggcctgc
      301 gcactctccg gcgccgacga gcagcccccg ttccaccccg agccgcggaa gtcggactgc
      361 gcgcaggaca agagcgtgtt cggcgagaca gaagagcaga agtgctccac tccggtctac
      421 ccgtggatgc agcggatgaa ttcgtgcaac agttcctcct ttgggcccag cggccggcga
      481 ggccgccaga catacacacg ttaccagacg ctggagctgg agaaggagtt tcactacaat
      541 cgctacctga cgcggcggcg gcgcatcgag atcgcgcacg ccctgtgcct gacggagagg
      601 cagatcaaga tatggttcca gaaccgacgc atgaagtgga aaaaggagag caaactgctc
      661 agcgcgtctc agctcagtgc cgaggaggag gaagaaaaac aggccgagtg cccaactttc
      721 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
      781 acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgaagccca gtgtgtcacc acacttcc
//
