LOCUS       sequence-285390-        1727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2949
FEATURES             Location/Qualifiers
     source          1..1727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1559..1583)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1591..1632
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccggcggc cgcggtgcag
       61 gaagcggtcg gcgtgtgctc ctacgggatg cagctcagct gggacatcaa cgatccgcag
      121 atgcctcagg agctggccct ctttgaccaa ttccgagagt ggcctgacgg ctatgtgcgc
      181 ttcatctaca gcagcgatga gaagaaggca cagcgtcacc tgagcggctg ggccatgcgc
      241 aacaccaaca accacaatgg ccacatcctc aagaagtcgt gcctgggtgt ggtggtgtgt
      301 acacaggcct gcaccctgcc cgacggttcc cgcctgcagc tgaggccggc catctgcgac
      361 aaggcacggc tgaaacagca gaagaaggca tgccctaact gtcattctgc tttggagttg
      421 attccttgtc gagggcacag cggatacccc gtaaccaact tttggcggct tgatggcaac
      481 gcgatctttt ttcaggccaa gggagttcat gatcatccaa gaccagagag caaatcagag
      541 acagaagcta gaagaagcgc catcaagaga caaatggcct ctttctacca accccagaaa
      601 aagagaattc gagaatccga ggcagaagaa aatcaagaca gcagtggtca tttcagcaac
      661 atacctccct tggaaaatcc agaagacttt gatatagtta ctgaaaccag cttccctatt
      721 ccagggcagc cttgcccttc cttcccaaag tctgatgttt acaaagctac ctgtgaccta
      781 gccacctttc aaggagacaa aatgccaccc ttccagaaat actcaagccc aagaatctat
      841 ttgcctaggc caccttgcag ctatgaattg gcaaaccctg gttatacaaa ttcaagccca
      901 tatcccaccc tttataagga ttccaccagt atccctaatg acacagactg ggttcatctg
      961 aacacactac aatgtaatgt caattcatac agcagctatg agagaagctt tgatttcacc
     1021 aacaaacagc atggctggaa accagctctt ggaaaaccca gccttgtgga aaggactaac
     1081 catgggcagt ttcaggccat ggccactcgc ccttattata acccagagct tccctgcagg
     1141 tacctcacga ctccaccacc aggtgcccct gccctacaaa ccgtgatcac caccaccact
     1201 aaagtgtcct accaggccta ccagccccct gctatgaaat acagtgacag tgtgcgagag
     1261 gtgaagagcc tttcgagctg taactatgct cctgaagata ctgggatgtc tgtctatcca
     1321 gaaccctggg gtcctccggt gacagtcacc agggcagcct ctccttcagg gccacctcct
     1381 atgaaaattg caggagattg ccgggccatc agacccactg tggctattcc ccacgagcca
     1441 gtttcctcta ggacagatga agcagagact tgggatgtgt gtctgtctgg gctgggctcc
     1501 gcagtcagtt actcagacag agtgggtccc ttctttacct acaacaatga ggattttttg
     1561 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1621 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1681 tatatatctt gtggaaagga cgatatcttc atcgttgtcg ttgcagg
//
