LOCUS       sequence-285393-        1175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2950
FEATURES             Location/Qualifiers
     source          1..1175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             427..450
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             520..537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(1007..1031)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1039..1080
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg ccgcggagct gagcatgggg
       61 ccagagctgc ccaccagccc gctggccatg gagtatgtca acgacttcga cctgctcaag
      121 ttcgacgtga agaaggagcc actggggcgc gcggagcgtc cgggcaggcc ctgcacacgc
      181 ctgcagccag ccggctcggt gtcctccaca ccgctcagca ctccgtgtag ctccgtgccc
      241 tcgtcgccca gcttcagccc gaccgaacag aagacacacc tcgaggatct gtactggatg
      301 gcgagcaact accagcagat gaaccccgag gcgctcaacc tgacgcccga ggacgcggtg
      361 gaagcgctca tcggctcgca cccagtgcca cagccgctgc aaagcttcga cagctttcgc
      421 ggcgctcacc accaccacca tcaccaccac cctcacccgc accacgcgta cccgggcgcc
      481 ggcgtggccc acgacgagct gggcccgcac gctcacccgc accatcacca tcatcaccaa
      541 gcgtcgccgc cgccgtccag cgccgctagc ccggcgcaac agctgcccac tagccacccc
      601 gggcccgggc cgcacgcgac ggcctcggcg acggcggcgg gcggcaacgg cagcgtggag
      661 gaccgcttct ccgatgacca gctcgtgtcc atgtccgtgc gcgagctgaa ccgccacctg
      721 cggggcttca ccaaggacga ggtgatccgc ctgaagcaga agcggcggac cctgaagaac
      781 cggggctacg cccagtcttg caggtataaa cgcgtccagc agaagcacca cctggagaat
      841 gagaagacgc agctcattca gcaggtggag cagcttaagc aggaggtgtc ccggctggcc
      901 cgcgagagag acgcctacaa ggtcaagtgc gagaaactcg ccaactccgg cttcagggag
      961 gcgggctcca ccagcgacag cccctcctct cccgagttct ttctgtgccc aactttcttg
     1021 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     1081 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1141 ggaaaggacg acccacggcc atcgggttac aaaca
//
