LOCUS       sequence-285396-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2951
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(518..542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             550..591
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aggagggctc cagctcgccc
       61 gtgtcccccg tggacagcct gggcaccagc gaggaggagc tcgagaggca gcccaagcgc
      121 ttcggccgga agcggcgcta cagcaagaag tcgagcgaag atggcagccc gaccccgggc
      181 aagcgcggca agaagggcag ccccagcgcg cagtccttcg aggagctgca gagccagcgc
      241 atcctggcca acgtgcgcga gcgccagcgc acccagtcgc tcaacgaggc cttcgcggcg
      301 ctgcgcaaga tcatccccac gctgccctct gacaagctga gcaagatcca gacgctcaag
      361 ctggccgcca ggtacataga cttcctctac caggtcctgc agagcgacga gatggacaat
      421 aagatgacca gctgcagcta cgtggcccac gagcgcctca gctacgcctt ctccgtgtgg
      481 cgcatggagg gcgcgtggtc catgtccgcc tcccactacc caactttctt gtacaaagtg
      541 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      601 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      661 gaggcgatag tggcggatac ctgcgc
//
