LOCUS       sequence-285405-        1439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2954
FEATURES             Location/Qualifiers
     source          1..1439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1271..1295)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1303..1344
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatgaatac cacagacaac
       61 ggtgtcaact gtctgtgtgc tatctgtggg gacagagcaa caggaaaaca ctatggggca
      121 tccagctgtg atgggtgcaa gggtttcttc agacgcagca ttcgtaagag tcacgtttat
      181 tcttgcaggt tcagtcggca atgtgttgtt gacaaggaca aaaggaatca atgtagatat
      241 tgtcgattaa gaaagtgttt tagagcggga atgaaaaaag aagctgtaca aaatgaacgt
      301 gacagaataa gcaccagaag aagcacattt gatggcagca acatcccctc cattaacaca
      361 ctggcacaag ctgaagttcg gtctcgccag atctcagtct caagccctgg gtcaagcact
      421 gacataaacg ttaagaaaat tgcaagtatt ggtgatgtct gtgaatctat gaaacagcag
      481 ctcttagtct tggtggaatg ggctaaatat attcctgcct tctgtgaatt accattggat
      541 gatcaggtgg cactgttgag agctcacgca ggggagcact tactgcttgg agctacaaag
      601 agatccatga tgtataaaga tattttgctt ttgggaaaca actatgttat tcaccgcaac
      661 agctgtgaag ttgagattag ccgtgtggcc aatcgtgttc tagatgagct ggttagacca
      721 tttcaagaaa tccagattga tgacaatgag tatgcttgtt taaaggcaat tgtatttttt
      781 gatccagatg caaaagggct aagcgatcca gtaaaaatta agaacatgag gttccaagtg
      841 cagatcggtt tggaggacta catcaatgat cggcagtatg actcccgggg gaggtttgga
      901 gagttgcttc tgctcctgcc cacactgcag agcatcacgt ggcaaatgat tgagcaaata
      961 cagtttgtta aactttttgg gatggttaaa attgacaatc tacttcagga aatgctatta
     1021 ggtggggctt ccaatgatgg cagtcatctc catcatccaa tgcatccaca tttgtctcaa
     1081 gacccattaa ctggacaaac tatactttta ggtcccatgt caacactggt tcatgcagac
     1141 cagatctcaa ctcctgaaac cccactccct tccccaccac aaggctctgg gcaagaacag
     1201 tacaaaatag ctgcaaacca agcatcagtc atttcacacc agcatctctc caaacaaaag
     1261 caattgtgag acccagcttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1321 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1381 atttcttggc tttatatatc ttgtggaaag gacgaaggaa actgaacgca aatgattcg
//
