LOCUS       sequence-285408-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2955
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(989..1013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1021..1062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt cccacggccc caagcagccc
       61 ggcgcggccg ccgcgccggc gggcggcaag gctccgggcc agcatggggg cttcgtggtg
      121 actgtcaagc aagagcgcgg cgagggtcca cgcgcgggcg agaaggggtc ccacgaggag
      181 gagccggtga agaaacgcgg ctggcccaag ggcaagaagc ggaagaagat tctgccgaat
      241 gggcccaagg caccggtcac gggctacgtg cgcttcctga acgagcggcg cgagcagatc
      301 cgcacgcgcc acccggatct gccctttccc gagatcacca agatgctggg cgccgagtgg
      361 agcaagctgc agccaacgga aaagcagcgg tacctggatg aggccgagag agagaagcag
      421 cagtacatga aggagctgcg ggcgtaccag cagtctgaag cctataagat gtgcacggag
      481 aagatccagg agaagaagat caagaaagaa gactcgagct ctgggctcat gaacactctc
      541 ctgaatggac acaagggtgg ggactgcgat ggcttctcca ccttcgatgt tcccatcttc
      601 actgaagagt tcttggacca aaacaaagcg cgtgaggcgg agcttcggcg cttgcggaag
      661 atgaatgtgg ccttcgagga gcagaacgcg gtactgcaga ggcacacgca gagcatgagc
      721 agcgcgcgcg agcgtctgga gcaggagctg gcgctggagg agcggaggac gctggcgctg
      781 cagcagcagc tccaggccgt gcgccaggcg ctcaccgcca gcttcgcctc actgccggtg
      841 ccgggcacgg gcgaaacgcc cacgctgggc actctggact tctacatggc ccggcttcac
      901 ggagccatcg agcgcgaccc cgcccagcac gagaagctca tcgtccgcat caaggaaatc
      961 ctggcccagg tcgccagcga gcacctgtgc ccaactttct tgtacaaagt ggttgatatc
     1021 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1081 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatcttgtg
     1141 tctttccgta gtgtcga
//
