LOCUS       sequence-285411-        1460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2956
FEATURES             Location/Qualifiers
     source          1..1460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1292..1316)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1324..1365
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcgactctc caaaaccctc
       61 gtcgacatgg acatggccga ctacagtgct gcactggacc cagcctacac caccctggaa
      121 tttgagaatg tgcaggtgtt gacgatgggc aatgacacgt ccccatcaga aggcaccaac
      181 ctcaacgcgc ccaacagcct gggtgtcagc gccctgtgtg ccatctgcgg ggaccgggcc
      241 acgggcaaac actacggtgc ctcgagctgt gacggctgca agggcttctt ccggaggagc
      301 gtgcggaaga accacatgta ctcctgcaga tttagccggc agtgcgtggt ggacaaagac
      361 aagaggaacc agtgccgcta ctgcaggctc aagaaatgct tccgggctgg catgaagaag
      421 gaagccgtcc agaatgagcg ggaccggatc agcactcgaa ggtcaagcta tgaggacagc
      481 agcctgccct ccatcaatgc gctcctgcag gcggaggtcc tgtcccgaca gatcacctcc
      541 cccgtctccg ggatcaacgg cgacattcgg gcgaagaaga ttgccagcat cgcagatgtg
      601 tgtgagtcca tgaaggagca gctgctggtt ctcgttgagt gggccaagta catcccagct
      661 ttctgcgagc tccccctgga cgaccaggtg gccctgctca gagcccatgc tggcgagcac
      721 ctgctgctcg gagccaccaa gagatccatg gtgttcaagg acgtgctgct cctaggcaat
      781 gactacattg tccctcggca ctgcccggag ctggcggaga tgagccgggt gtccatacgc
      841 atccttgacg agctggtgct gcccttccag gagctgcaga tcgatgacaa tgagtatgcc
      901 tacctcaaag ccatcatctt ctttgaccca gatgccaagg ggctgagcga tccagggaag
      961 atcaagcggc tgcgttccca ggtgcaggtg agcttggagg actacatcaa cgaccgccag
     1021 tatgactcgc gtggccgctt tggagagctg ctgctgctgc tgcccacctt gcagagcatc
     1081 acctggcaga tgatcgagca gatccagttc atcaagctct tcggcatggc caagattgac
     1141 aacctgttgc aggagatgct gctgggaggt ccgtgccaag cccaggaggg gcggggttgg
     1201 agtggggact ccccaggaga caggcctcac acagtgagct cacccctcag ctccttggct
     1261 tccccactgt gccgctttgg gcaagttgct ttgccaactt tcttgtacaa agtggttgat
     1321 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1381 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaagca
     1441 cactgacagt ataaccggag
//
