LOCUS       sequence-285414-        1637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2957
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1469..1493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1501..1542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatgcgact ctccaaaacc
       61 ctcgtcgaca tggacatggc cgactacagt gctgcactgg acccagccta caccaccctg
      121 gaatttgaga atgtgcaggt gttgacgatg ggcaatgaca cgtccccatc agaaggcacc
      181 aacctcaacg cgcccaacag cctgggtgtc agcgccctgt gtgccatctg cggggaccgg
      241 gccacgggca aacactacgg tgcctcgagc tgtgacggct gcaagggctt cttccggagg
      301 agcgtgcgga agaaccacat gtactcctgc agatttagcc ggcagtgcgt ggtggacaaa
      361 gacaagagga accagtgccg ctactgcagg ctcaagaaat gcttccgggc tggcatgaag
      421 aaggaagccg tccagaatga gcgggaccgg atcagcactc gaaggtcaag ctatgaggac
      481 agcagcctgc cctccatcaa tgcgctcctg caggcggagg tcctgtcccg acagatcacc
      541 tcccccgtct ccgggatcaa cggcgacatt cgggcgaaga agattgccag catcgcagat
      601 gtgtgtgagt ccatgaagga gcagctgctg gttctcgttg agtgggccaa gtacatccca
      661 gctttctgcg agctccccct ggacgaccag gtggccctgc tcagagccca tgctggcgag
      721 cacctgctgc tcggagccac caagagatcc atggtgttca aggacgtgct gctcctaggc
      781 aatgactaca ttgtccctcg gcactgcccg gagctggcgg agatgagccg ggtgtccata
      841 cgcatccttg acgagctggt gctgcccttc caggagctgc agatcgatga caatgagtat
      901 gcctacctca aagccatcat cttctttgac ccagatgcca aggggctgag cgatccaggg
      961 aagatcaagc ggctgcgttc ccaggtgcag gtgagcttgg aggactacat caacgaccgc
     1021 cagtatgact cgcgtggccg ctttggagag ctgctgctgc tgctgcccac cttgcagagc
     1081 atcacctggc agatgatcga gcagatccag ttcatcaagc tcttcggcat ggccaagatt
     1141 gacaacctgt tgcaggagat gctgctggga gggtccccca gcgatgcacc ccatgcccac
     1201 caccccctgc accctcacct gatgcaggaa catatgggaa ccaacgtcat cgttgccaac
     1261 acaatgccca ctcacctcag caacggacag atgtgtgagt ggccccgacc caggggacag
     1321 gcagccaccc ctgagacccc acagccctca ccgccaggtg gctcagggtc tgagccctat
     1381 aagctcctgc cgggagccgt cgccacaatc gtcaagcccc tctctgccat cccccagccg
     1441 accatcacca agcaggaagt tatctgagac ccagctttct tgtacaaagt ggttgatatc
     1501 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacagaggc
     1621 ttcaaggcca tacacca
//
