LOCUS       sequence-285417-        2567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2958
FEATURES             Location/Qualifiers
     source          1..2567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2399..2423)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2431..2472
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgagcgacca agatcactcc
       61 atggatgaaa tgacagctgt ggtgaaaatt gaaaaaggag ttggtggcaa taatgggggc
      121 aatggtaatg gtggtggtgc cttttcacag gctcgaagta gcagcacagg cagtagcagc
      181 agcactggag gaggagggca ggagtcccag ccatcccctt tggctctgct ggcagcaact
      241 tgcagcagaa ttgagtcacc caatgagaac agcaacaact cccagggccc gagtcagtca
      301 gggggaacag gtgagcttga cctcacagcc acacaacttt cacagggtgc caatggctgg
      361 cagatcatct cttcctcctc tggggctacc cctacctcaa aggaacagag tggcagcagt
      421 accaatggca gcaatggcag tgagtcttcc aagaatcgca cagtctctgg tgggcagtat
      481 gttgtggctg ccgctcccaa cttacagaac cagcaagttc tgacaggact acctggagtg
      541 atgcctaata ttcagtatca agtaatccca cagttccaga ccgttgatgg gcaacagctg
      601 cagtttgctg ccactggggc ccaagtgcag caggatggtt ctggtcaaat acagatcata
      661 ccaggtgcaa accaacagat tatcacaaat cgaggaagtg gaggcaacat cattgctgct
      721 atgccaaacc tactccagca ggctgtcccc ctccaaggcc tggctaataa tgtactctca
      781 ggacagactc agtatgtgac caatgtacca gtggccctga atgggaacat caccttgcta
      841 cctgtcaaca gcgtttctgc agctaccttg actcccagct ctcaggcagt cacgatcagc
      901 agctctgggt cccaggagag tggctcacag cctgtcacct cagggactac catcagttct
      961 gccagcttgg tatcatcaca agccagttcc agctcctttt tcaccaatgc caatagctac
     1021 tcaactacta ctaccaccag caacatggga attatgaact ttactaccag tggatcatca
     1081 gggaccaact ctcaaggcca gacaccccag agggtcagtg ggctacaggg gtctgatgct
     1141 ctgaacatcc agcaaaacca gacatctgga ggctcattgc aagcaggcca gcaaaaagaa
     1201 ggagagcaaa accagcagac acagcagcaa caaattctta tccagcctca gctagttcaa
     1261 gggggacagg ccctccaggc cctccaagca gcaccattgt cagggcagac ctttacaact
     1321 caagccatct cccaggaaac cctccagaac ctccagcttc aggctgttcc aaactctggt
     1381 cccatcatca tccggacacc aacagtgggg cccaatggac aggtcagttg gcagactcta
     1441 cagctgcaga acctccaagt tcagaaccca caagcccaaa caatcacctt agccccaatg
     1501 cagggtgttt ccttggggca gaccagcagc agcaacacca ctctcacacc cattgcctca
     1561 gctgcttcca ttcctgctgg cacagtcact gtgaatgctg ctcaactctc ctccatgcca
     1621 ggcctccaga ccattaacct cagtgcattg ggtacttcag gaatccaggt gcacccaatt
     1681 caaggcctgc cgttggctat agcaaatgcc ccaggtgatc atggagctca gcttggtctc
     1741 catggggctg gtggtgatgg aatacatgat gacacagcag gtggagagga aggagaaaac
     1801 agcccagatg cccaacccca agccggtcgg aggacccggc gggaagcatg cacctgcccc
     1861 tactgtaaag acagtgaagg aaggggctcg ggggatcctg gcaaaaagaa acagcatatt
     1921 tgccacatcc aaggctgtgg gaaagtgtat ggcaagacct ctcacctgcg ggcacacttg
     1981 cgctggcata caggcgagag gccatttatg tgtacctggt catactgtgg gaaacgcttc
     2041 acacgttcgg atgagctaca gaggcacaaa cgtacacaca caggtgagaa gaaatttgcc
     2101 tgccctgagt gtcctaagcg cttcatgagg agtgaccacc tgtcaaaaca tatcaagacc
     2161 caccagaata agaagggagg cccaggtgta gctctgagtg tgggcacttt gcccctggac
     2221 agtggggcag gttcagaagg cagtggcact gccactcctt cagcccttat taccaccaat
     2281 atggtagcca tggaggccat ctgtccagag ggcattgccc gtcttgccaa cagtggcatc
     2341 aacgtcatgc aggtggcaga tctgcagtcc attaatatca gtggcaatgg cttctgagac
     2401 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2461 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2521 tatatatctt gtggaaagga cgaagagtaa taagttctaa gtcatct
//
