LOCUS       sequence-285420-        2723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2959
FEATURES             Location/Qualifiers
     source          1..2723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2555..2579)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2587..2628
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggcggaacc ttcccaggcc
       61 ccgaccccgg ccccggctgc gcagccccgg ccccttcagt ccccagcccc tgccccaact
      121 ccgactcctg cacccagccc ggcttcagcc ccgattccga ctcccacccc ggcaccagcc
      181 cctgccccag ctgcagcccc agccggcagc acagggactg gggggcccgg ggtaggaagt
      241 gggggggccg ggagcggggg ggatccggct cgacctggcc tgagccagca gcagcgcgcc
      301 agtcagagga aggcgcaagt ccgggggctg ccgcgcgcca agaagcttga gaagctaggg
      361 gtcttctcgg cttgcaaggc caatgaaacc tgtaagtgta atggctggaa aaaccccaag
      421 ccccccactg caccccgcat ggatctgcag cagccagctg ccaacctgag tgagctgtgc
      481 cgcagttgtg agcacccctt ggctgaccac gtatcccact tggagaatgt gtcagaggat
      541 gagataaacc gactgctggg gatggtggtg gatgtggaga atctcttcat gtctgttcac
      601 aaggaagagg acacagacac caagcaggtc tatttctacc tcttcaagct actgcggaaa
      661 tgcatcctgc agatgacccg gcctgtggtg gaggggtccc tgggcagccc tccatttgag
      721 aaacctaata ttgagcaggg tgtgctgaac tttgtgcagt acaagtttag tcacctggct
      781 ccccgggagc ggcagacgat gttcgagctc tcaaagatgt tcttgctctg ccttaactac
      841 tggaagcttg agacacctgc ccagtttcgg cagaggtctc aggctgagga cgtggctacc
      901 tacaaggtca attacaccag atggctctgt tactgccacg tgccccagag ctgtgatagc
      961 ctcccccgct acgaaaccac tcatgtcttt gggcgaagcc ttctccggtc cattttcacc
     1021 gttacccgcc ggcagctgct ggaaaagttc cgagtggaga aggacaaatt ggtgcccgag
     1081 aagaggaccc tcatcctcac tcacttcccc aaattcctgt ccatgctgga ggaggagatc
     1141 tatggggcaa actctccaat ctgggagtca ggcttcacca tgccaccctc agaggggaca
     1201 cagctggttc cccggccagc ttcagtcagt gcagcggttg ttcccagcac ccccatcttc
     1261 agccccagca tgggtggggg cagcaacagc tccctgagtc tggattctgc aggggccgag
     1321 cctatgccag gcgagaagag gacgctccca gagaacctga ccctggagga tgccaagcgg
     1381 ctccgtgtga tgggtgacat ccccatggag ctggtcaatg aggtcatgct gaccatcact
     1441 gaccctgctg ccatgctggg gcctgagacg agcctgcttt cggccaatgc ggcccgggat
     1501 gagacagccc gcctggagga gcgccgcggc atcatcgagt tccatgtcat cggcaactca
     1561 ctgacgccca aggccaaccg gcgggtgttg ctgtggctcg tggggctgca gaatgtcttt
     1621 tcccaccagc tgccgcgcat gcctaaggag tatatcgccc gcctcgtctt tgacccgaag
     1681 cacaagactc tggccttgat caaggatggg cgggtcatcg gtggcatctg cttccgcatg
     1741 tttcccaccc agggcttcac ggagattgtc ttctgtgctg tcacctcgaa tgagcaggtc
     1801 aagggttatg ggacccacct gatgaaccac ctgaaggagt atcacatcaa gcacaacatt
     1861 ctctacttcc tcacctacgc cgacgagtac gccatcggct acttcaaaaa gcagggtttc
     1921 tccaaggaca tcaaggtgcc caagagccgc tacctgggct acatcaagga ctacgaggga
     1981 gcgacgctga tggagtgtga gctgaatccc cgcatcccct acacggagct gtcccacatc
     2041 atcaagaagc agaaagagat catcaagaag ctgattgagc gcaaacaggc ccagatccgc
     2101 aaggtctacc cggggctcag ctgcttcaag gagggcgtga ggcagatccc tgtggagagc
     2161 gttcctggca ttcgagagac aggctggaag ccattgggga aggagaaggg gaaggagctg
     2221 aaggaccccg accagctcta cacaaccctc aaaaacctgc tggcccaaat caagtctcac
     2281 cccagtgcct ggcccttcat ggagcctgtg aagaagtcgg aggcccctga ctactacgag
     2341 gtcatccgct tccccattga cctgaagacc atgactgagc ggctgcgaag ccgctactac
     2401 gtgacccgga agctctttgt ggccgacctg cagcgggtca tcgccaactg tcgcgagtac
     2461 aaccccccgg acagcgagta ctgccgctgt gccagcgccc tggagaagtt cttctacttc
     2521 aagctcaagg agggaggcct cattgacaag taggacccag ctttcttgta caaagtggtt
     2581 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     2641 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     2701 gttttaacct catgttacaa agt
//
