LOCUS       sequence-285426-        1607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2961
FEATURES             Location/Qualifiers
     source          1..1607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1439..1463)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1471..1512
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg caaccataga agaaattgca
       61 catcaaatta ttgaacaaca gatgggagag attgttacag agcagcaaac tgggcagaaa
      121 atccagattg tgacagcact tgatcataat acccaaggca agcagttcat tctgacaaat
      181 cacgacggct ctactccaag caaagtcatt ctggccaggc aagattccac tccgggaaaa
      241 gttttcctta caactccaga tgcagcaggt gtcaaccagt tattttttac cactcctgat
      301 ctgtctgcac aacacctgca gctcctaaca gataattctc cagaccaagg accaaataag
      361 gtttttgatc tttgcgtagt atgtggagac aaagcatcag gacgtcatta tggagcagta
      421 acttgtgaag gctgcaaagg attttttaaa agaagcatcc gaaaaaattt agtatattca
      481 tgtcgaggat caaaggattg tattattaat aagcaccacc gaaaccgctg tcaatactgc
      541 aggttacaga gatgtattgc gtttggaatg aagcaagact ctgtccaatg tgaaagaaaa
      601 cccattgaag tatcacgaga aaaatcttcc aactgtgccg cttcaacaga aaaaatctat
      661 atccgaaagg accttcgtag cccattaact gcaactccaa cttttgtaac agatagtgaa
      721 agtacaaggt caacaggact gttagattca ggaatgttca tgaatattca tccatctgga
      781 gtaaaaactg agtcagctgt gctgatgaca tcagataagg ctgaatcatg tcagggagat
      841 ttaagtacat tggccaatgt ggttacatca ttagcgaatc ttggaaaaac taaagatctt
      901 tctcaaaata gtaatgaaat gtctatgatt gaaagcttaa gcaatgatga tacctctttg
      961 tgtgaatttc aagaaatgca gaccaacggt gatgtttcaa gggcatttga cactcttgca
     1021 aaagcattga atcctggaga gagcacagcc tgccagagct cagtagcggg catggaagga
     1081 agtgtacacc taatcactgg agattcaagc ataaattaca ccgaaaaaga ggggccactt
     1141 ctcagcgatt cacatgtagc tttcaggctc accatgcctt ctcctatgcc tgagtacctg
     1201 aatgtgcact acattgggga gtctgcctcc agactgctgt tcttatcaat gcactgggca
     1261 ctttcgattc cttctttcca ggctctaggg caagaaaaca gcatatcact ggtgaaagct
     1321 tactggaatg aactttttac tcttggtctt gcccagtgct ggcaagtgat gaatgtagca
     1381 actatattag caacatttgt caattgtctt cacaatagtc ttcaacaagc agaggggtac
     1441 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1501 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1561 tatatatctt gtggaaagga cgagtagggg ttcaccgaaa tctacac
//
