LOCUS       sequence-285432-        2060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2963
FEATURES             Location/Qualifiers
     source          1..2060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1892..1916)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1924..1965
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatgaccag cccctcccca
       61 cgcatccaga taatctccac cgactctgct gtagcctcac ctcagcgcat tcagggctct
      121 gaacctgcct ctggcccatt gagtgttttc acatctttga acaaagagaa gattgtcaca
      181 gaccagcaga caggacagaa aatccagata gtcaccgcag tggacgcctc cggatccccc
      241 aaacagcagt tcatcctgac cagcccagat ggagctggaa ctgggaaggt gatcctggct
      301 tccccagaga catccagcgc caagcaactc atattcacca cctcagacaa cctcgtccct
      361 ggcaggatcc agattgtcac ggattctgcc tctgtggagc gtttactggg gaagacggac
      421 gtccagcggc cccaggtggt agagtactgt gtggtctgtg gcgacaaagc ctccggccgt
      481 cactatgggg ctgtcagttg tgaaggttgc aaaggtttct tcaaaaggag tgtgaggaaa
      541 aatttgacct acagctgccg gagcaaccaa gactgcatca tcaataaaca tcaccggaac
      601 cgctgtcagt tttgccggct gaaaaaatgc ttagagatgg gcatgaaaat ggaatctgtg
      661 cagagtgaac ggaagccctt cgatgtgcaa cgggagaaac caagcaattg tgctgcttca
      721 actgagaaaa tctatatccg gaaagacctg agaagtcccc tgatagctac tcccacgttt
      781 gtggcagaca aagatggagc aagacaaaca ggtcttcttg atccagggat gcttgtgaac
      841 atccagcagc ctttgatacg tgaggatggt acagttctcc tggccacgga ttctaaggct
      901 gaaacaagcc agggagctct gggcacactg gcaaatgtag tgacctccct tgccaaccta
      961 agtgaatctt tgaacaacgg tgacacttca gaaatccagc cagaggacca gtctgcaagt
     1021 gagataactc gggcatttga taccttagct aaagcactta ataccacaga cagctcctct
     1081 tctccaagct tggcagatgg gatagacacc agtggaggag ggagcatcca cgtcatcagc
     1141 agagaccagt cgacacccat cattgaggtt gaaggccccc tcctttcaga cacacacgtc
     1201 acatttaagc taacaatgcc cagtccaatg ccagagtacc tcaacgtgca ctacatctgt
     1261 gagtctgcat cccgtctgct tttcctctca atgcactggg ctcggtcaat cccagccttt
     1321 caggcacttg ggcaggactg caacaccagc cttgtgcggg cctgctggaa tgagctcttc
     1381 accctcggcc tggcccagtg tgcccaggtc atgagtctct ccaccatcct ggctgccatt
     1441 gtcaaccacc tgcagaacag catccaggaa gataaacttt ctggtgaccg gataaagcaa
     1501 gtcatggagc acatctggaa gctgcaggag ttctgtaaca gcatggcgaa gctggatata
     1561 gatggctatg agtatgcata ccttaaagct atagttctct ttagccccga tcatccaggt
     1621 ttgaccagca caagccagat tgaaaaattc caagaaaagg cacagatgga gttgcaggac
     1681 tatgttcaga aaacctactc agaagacacc taccgattgg cccggatcct cgttcgcctg
     1741 ccggcactca ggctgatgag ctccaacata acagaagaac ttttttttac tggtctcatt
     1801 ggcaatgttt cgatagacag cataatcccc tacatcctca agatggagac agcagagtat
     1861 aatggccaga tcaccggagc cagtctatga gacccagctt tcttgtacaa agtggttgat
     1921 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     1981 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagaat
     2041 tcccaacaaa ctgacatgca
//
