LOCUS       sequence-285435-        2159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2964
FEATURES             Location/Qualifiers
     source          1..2159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1991..2015)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2023..2064
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatggctac aaatatggag
       61 gggctggttc agcacagagt ggggacccag caggtggctg aggtaacacg tacacagacc
      121 tctcggccgg aatctccagg gatgaccagc ccctccccac gcatccagat aatctccacc
      181 gactctgctg tagcctcacc tcagcgcatt cagggctctg aacctgcctc tggcccattg
      241 agtgttttca catctttgaa caaagagaag attgtcacag accagcagac aggacagaaa
      301 atccagatag tcaccgcagt ggacgcctcc ggatccccca aacagcagtt catcctgacc
      361 agcccagatg gagctggaac tgggaaggtg atcctggctt ccccagagac atccagcgcc
      421 aagcaactca tattcaccac ctcagacaac ctcgtccctg gcaggatcca gattgtcacg
      481 gattctgcct ctgtggagcg tttactgggg aagacggacg tccagcggcc ccaggtggta
      541 gagtactgtg tggtctgtgg cgacaaagcc tccggccgtc actatggggc tgtcagttgt
      601 gaaggttgca aaggtttctt caaaaggagt gtgaggaaaa atttgaccta cagctgccgg
      661 agcaaccaag actgcatcat caataaacat caccggaacc gctgtcagtt ttgccggctg
      721 aaaaaatgct tagagatggg catgaaaatg gaatctgtgc agagtgaacg gaagcccttc
      781 gatgtgcaac gggagaaacc aagcaattgt gctgcttcaa ctgagaaaat ttatatccgg
      841 aaagacctga gaagtcccct gatagctact cccacgtttg tggcagacaa agatggagca
      901 agacaaacag gtcttcttga tccagggatg cttgtgaaca tccagcagcc tttgatacgt
      961 gaggatggta cagttctcct ggccacggat tctaaggctg aaacaagcca gggagctctg
     1021 ggcacactgg caaatgtagt gacctccctt gccaacctaa gtgaatcttt gaacaacggt
     1081 gacacttcag aaatccagcc agaggaccag tctgcaagtg agataactcg ggcatttgat
     1141 accttagcta aagcacttaa taccacagac agctcctctt ctccaagctt ggcagatggg
     1201 atagacacca gtggaggagg gagcatccac gtcatcagca gagaccagtc gacacccatc
     1261 attgaggttg aaggccccct cctttcagac acacacgtca catttaagct aacaatgccc
     1321 agtccaatgc cagagtacct caacgtgcac tacatctgtg agtctgcatc ccgtctgctt
     1381 ttcctctcaa tgcactgggc tcggtcaatc ccagcctttc aggcacttgg gcaggactgc
     1441 aacaccagcc ttgtgcgggc ctgctggaat gagctcttca ccctcggcct ggcccagtgt
     1501 gcccaggtca tgagtctctc caccatcctg gctgccattg tcaaccacct gcagaacagc
     1561 atccaggaag ataaactttc tggtgaccgg ataaagcaag tcatggagca catctggaag
     1621 ctgcaggagt tctgtaacag catggcgaag ctggatatag atggctatga gtatgcatac
     1681 cttaaagcta tagttctctt tagccccgat catccaggtt tgaccagcac aagccagatt
     1741 gaaaaattcc aagaaaaggc acagatggag ttgcaggact atgttcagaa aacctactca
     1801 gaagacacct accgattggc ccggatcctc gttcgcctgc cggcactcag gctgatgagc
     1861 tccaacataa cagaagaact tttttttact ggtctcattg gcaatgtttc gatagacagc
     1921 ataatcccct acatcctcaa gatggagaca gcagagtata atggccagat caccggagcc
     1981 agtctatgag acccagcttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     2041 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2101 atttcttggc tttatatatc ttgtggaaag gacgagtagt tgactttaca atatgtatt
//
