LOCUS       sequence-285441-        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2966
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1448..1472)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1480..1521
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgggcgagaa ccaccagtgg
       61 cagggcagca tcctctacaa catgcttatg agcgcgaagc aaacgcgcgc ggctcctgag
      121 gctccagaga cgcggctggt ggatcagtgc tggggctgtt cgtgcggcga tgagcccggg
      181 gtgggcagag aggggctgct gggcgggcgg aacgtggcgc tcctgtaccg ctgctgcttt
      241 tgcggtaaag accacccacg gcagggcagc atcctctaca gcatgctgac gagcgcaaag
      301 caaacgtacg cggcaccgaa ggcgcccgag gcgacgctgg gtccgtgctg gggctgttcg
      361 tgcggctctg atcccggggt gggcagagcg gggcttccgg gtgggcggcc cgtggcactc
      421 ctgtaccgct gctgcttttg tggtgaagac cacccgcggc agggcagcat cctctacagc
      481 ttgctcacta gctcaaagca aacgcacgtg gctccggcag cgcccgaggc acggccaggg
      541 ggcgcgtggt gggaccgctc ctacttcgcg cagaggccag ggggtaaaga ggcgctacca
      601 ggcgggcggg ccacggcgct tctgtaccgc tgctgctttt gcggtgaaga ccacccgcag
      661 cagggcagca ccctctactg cgtgcccacg agcacaaatc aagcgcaggc ggctccggag
      721 gagcggccga gggccccctg gtgggacacc tcctctggtg cgctgcggcc ggtggcgctc
      781 aagagtccac aggtggtctg cgaggcagcc tcagcgggcc tgttgaagac gctgcgcttc
      841 gtcaagtact tgccctgctt ccaggtgctg cccctggacc agcagctggt gctggtgcgc
      901 aactgctggg cgtccctgct catgcttgag ctggcccagg accgcttgca gttcgagact
      961 gtggaagtct cggagcccag catgctgcag aagatcctca ccaccaggcg gcgggagacc
     1021 gggggcaacg agccactgcc cgtgcccacg ctgcagcacc atttggcacc gccggcggag
     1081 gccaggaagg tgccctccgc ctcccaggtc caagccatca agtgctttct ttccaaatgc
     1141 tggagtctga acatcagtac caaggagtac gcctacctca aggggaccgt gctctttaac
     1201 ccggacgtgc cgggcctgca gtgcgtgaag tacattcagg gactccagtg gggaactcag
     1261 caaatactca gtgaacacac caggatgacg caccaagggc cccatgacag attcatcgaa
     1321 cttaatagta cccttttcct gctgagattc atcaatgcca atgtcattgc tgaactgttc
     1381 ttcaggccca tcatcggcac agtcagcatg gatgatatga tgctggaaat gctctgtaca
     1441 aagatatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1501 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1561 tcttggcttt atatatcttg tggaaaggac gaaacattcc gaccatccta ccggaa
//
