LOCUS       sequence-285447-        1622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2968
FEATURES             Location/Qualifiers
     source          1..1622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1454..1478)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1486..1527
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatggcggg cgagaaccac
       61 cagtggcagg gcagcatcct ctacaacatg cttatgagcg cgaagcaaac gcgcgcggct
      121 cctgaggctc cagagacgcg gctggtggat cagtgctggg gctgttcgtg cggcgatgag
      181 cccggggtgg gcagagaggg gctgctgggc gggcggaacg tggcgctcct gtaccgctgc
      241 tgcttttgcg gtaaagacca cccacggcag ggcagcatcc tctacagcat gctgacgagc
      301 gcaaagcaaa cgtacgcggc accgaaggcg cccgaggcga cgctgggtcc gtgctggggc
      361 tgttcgtgcg gctctgatcc cggggtgggc agagcggggc ttccgggtgg gcggcccgtg
      421 gcactcctgt accgctgctg cttttgtggt gaagaccacc cgcggcaggg cagcatcctc
      481 tacagcttgc tcactagctc aaagcaaacg cacgtggctc cggcagcgcc cgaggcacga
      541 ccagggggcg cgtggtggga ccgctcctac ttcgcgcaga ggccaggggg taaagaggcg
      601 ctaccaggcg ggcgggccac ggcgcttctg taccgctgct gcttttgcgg tgaagaccac
      661 ccgcagcagg gcagcaccct ctactgcgtg cccacgagca caaatcaagc gcaggcggct
      721 ccggaggagc ggccgagggc cccctggtgg gacacctcct ctggtgcgct gcggccggtg
      781 gcgctcaaga gtccacaggt ggtctgcgag gcagcctcag cgggcctgtt gaagacgctg
      841 cgcttcgtca agtacttgcc ctgcttccag gtgctgcccc tggaccagca gctggtgctg
      901 gtgcgcaact gctgggcgtc cctgctcatg cttgagctgg cccaggaccg cttgcagttc
      961 gagactgtgg aagtctcgga gcccagcatg ctgcagaaga tcctcaccac caggcggcgg
     1021 gagaccgggg gcaacgagcc actgcccgtg cccacgctgc agcaccattt ggcaccgccg
     1081 gcggaggcca ggaaggtgcc ctccgcctcc caggtccaag ccatcaagtg ctttctttcc
     1141 aaatgctgga gtctgaacat cagtaccaag gagtacgcct acctcaaggg gaccgtgctc
     1201 tttaacccgg acgtgccggg cctgcagtgc gtgaagtaca ttcagggact ccagtgggga
     1261 actcagcaaa tactcagtga acacaccagg atgacgcacc aagggcccca tgacagattc
     1321 atcgaactta atagtaccct tttcctgctg agattcatca atgccaatgt cattgctgaa
     1381 ctgttcttca ggcccatcat cggcacagtc agcatggatg atatgatgct ggaaatgctc
     1441 tgtacaaaga tagacccagc tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1501 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1561 tcgatttctt ggctttatat atcttgtgga aaggacgaac cttttgtgtg ctgggtaggt
     1621 tt
//
