LOCUS       sequence-285456-        1637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2971
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1469..1493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1501..1542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgggatcaaa aatgaatctc
       61 attgaacatt cccatttacc taccacagat gaattttctt tttctgaaaa tttatttggt
      121 gttttaacag aacaagtggc aggtcctctg ggacagaacc tggaagtgga accatactcg
      181 caatacagca atgttcagtt tccccaagtt caaccacaga tttcctcgtc atcctattat
      241 tccaacctgg gtttctaccc ccagcagcct gaagagtggt actctcctgg aatatatgaa
      301 ctcaggcgta tgccagctga gactctctac cagggagaaa ctgaggtagc agagatgcct
      361 gtaacaaaga agccccgcat gggcgcgtca gcagggagga tcaaagggga tgagctgtgt
      421 gttgtttgtg gagacagagc ctctggatac cactataatg cactgacctg tgaggggtgt
      481 aaaggtttct tcaggagaag cattaccaaa aacgctgtgt acaagtgtaa aaacgggggc
      541 aactgtgtga tggatatgta catgcgaaga aagtgtcaag agtgtcgact aaggaaatgc
      601 aaagagatgg gaatgttggc tgaatgtatg tatacaggct tgttaactga aattcagtgt
      661 aaatctaagc gactgagaaa aaatgtgaag cagcatgcag atcagaccgt gaatgaagac
      721 agtgaaggtc gtgacttgcg acaagtgacc tcgacaacaa agtcatgcag ggagaaaact
      781 gaactcaccc cagatcaaca gactcttcta cattttatta tggattcata taacaaacag
      841 aggatgcctc aggaaataac aaataaaatt ttaaaagaag aattcagtgc agaagaaaat
      901 tttctcattt tgacggaaat ggcaaccaat catgtacagg ttcttgtaga attcacaaaa
      961 aagctaccag gatttcagac tttggaccat gaagaccaga ttgctttgct gaaagggtct
     1021 gcggttgaag ctatgttcct tcgttcagct gagattttca ataagaaact tccgtctggg
     1081 cattctgacc tattggaaga aagaattcga aatagtggta tctctgatga atatataaca
     1141 cctatgttta gtttttataa aagtattggg gaactgaaaa tgactcaaga ggagtatgct
     1201 ctgcttacag caattgttat cctgtctcca gatagacaat acataaagga tagagaggca
     1261 gtagagaagc ttcaggagcc acttcttgat gtgctacaaa agttgtgtaa gattcaccag
     1321 cctgaaaatc ctcaacactt tgcctgtctc ctgggtcgcc tgactgaatt acggacattc
     1381 aatcatcacc acgctgagat gctgatgtca tggagagtaa acgaccacaa gtttacccca
     1441 cttctctgtg aaatctggga cgtgcagttg ccaactttct tgtacaaagt ggttgatatc
     1501 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacctgcac
     1621 ggctattcgt gcaaagc
//
