LOCUS       sequence-285459-        1631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2972
FEATURES             Location/Qualifiers
     source          1..1631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1463..1487)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1495..1536
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatgggatc aaaaatgaat
       61 ctcattgaac attcccattt acctaccaca gatgaatttt ctttttctga aaatttattt
      121 ggtgttttaa cagaacaagt ggcaggtcct ctgggacaga acctggaagt ggaaccatac
      181 tcgcaataca gcaatgttca gtttccccaa gttcaaccac agatttcctc gtcatcctat
      241 tattccaacc tgggtttcta cccccagcag cctgaagagt ggtactctcc tggaatatat
      301 gaactcaggc gtatgccagc tgagactctc taccagggag aaactgaggt agcagagatg
      361 cctgtaacaa agaagccccg catgggcgcg tcagcaggga ggatcaaagg ggatgagctg
      421 tgtgttgttt gtggagacag agcctctgga taccactata atgcactgac ctgtgagggg
      481 tgtaaaggtt tcttcaggag aagcattacc aaaaacgctg tgtacaagtg taaaaacggg
      541 ggcaactgtg tgatggatat gtacatgcga agaaagtgtc aagagtgtcg actaaggaaa
      601 tgcaaagaga tgggaatgtt ggctgaatgc ttgttaactg aaattcagtg taaatctaag
      661 cgactgagaa aaaatgtgaa gcagcatgca gatcagaccg tgaatgaaga cagtgaaggt
      721 cgtgacttgc gacaagtgac ctcgacaaca aagtcatgca gggagaaaac tgaactcacc
      781 ccagatcaac agactcttct acattttatt atggattcat ataacaaaca gaggatgcct
      841 caggaaataa caaataaaat tttaaaagaa gaattcagtg cagaagaaaa ttttctcatt
      901 ttgacggaaa tggcaaccaa tcatgtacag gttcttgtag aattcacaaa aaagctacca
      961 ggatttcaga ctttggacca tgaagaccag attgctttgc tgaaagggtc tgcggttgaa
     1021 gctatgttcc ttcgttcagc tgagattttc aataagaaac ttccgtctgg gcattctgac
     1081 ctattggaag aaagaattcg aaatagtggt atctctgatg aatatataac acctatgttt
     1141 agtttttata aaagtattgg ggaactgaaa atgactcaag aggagtatgc tctgcttaca
     1201 gcaattgtta tcctgtctcc agatagacaa tacataaagg atagagaggc agtagagaag
     1261 cttcaggagc cacttcttga tgtgctacaa aagttgtgta agattcacca gcctgaaaat
     1321 cctcaacact ttgcctgtct cctgggtcgc ctgactgaat tacggacatt caatcatcac
     1381 cacgctgaga tgctgatgtc atggagagta aacgaccaca agtttacccc acttctctgt
     1441 gaaatctggg acgtgcagtg agacccagct ttcttgtaca aagtggttga tatcggtaag
     1501 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaat gacccccaga
     1621 gagcgcttgt c
//
