LOCUS       sequence-285462-        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2973
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1121..1145)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1153..1194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg agcagccaca ggaggaagcc
       61 cctgaggtcc gggaagagga ggagaaagag gaagtggcag aggcagaagg agccccagag
      121 ctcaatgggg gaccacagca tgcacttcct tccagcagct acacagacct ctcccggagc
      181 tcctcgccac cctcactgct ggaccaactg cagatgggct gtgacggggc ctcatgcggc
      241 agcctcaaca tggagtgccg ggtgtgcggg gacaaggcat cgggcttcca ctacggtgtt
      301 catgcatgtg aggggtgcaa gggcttcttc cgtcgtacga tccgcatgaa gctggagtac
      361 gagaagtgtg agcgcagctg caagattcag aagaagaacc gcaacaagtg ccagtactgc
      421 cgcttccaga agtgcctggc actgggcatg tcacacaacg ctatccgttt tggtcggatg
      481 ccggaggctg agaagaggaa gctggtggca gggctgactg caaatgaggg gagccagtac
      541 aacccacagg tggccgacct gaaggccttc tccaagcaca tctacaatgc ctacctgaaa
      601 aacttcaaca tgaccaaaaa gaaggcccgc agcatcctca ccggcaaagc cagccacacg
      661 gcgccctttg tgatccacga catcgagaca ttgtggcagg cagagaaggg gctggtgtgg
      721 aagcagttgg tgaatggcct gcctccctac aaggagatca gcgtgcacgt cttctaccgc
      781 tgccagtgca ccacagtgga gaccgtgcgg gagctcactg agttcgccaa gagcatcccc
      841 agcttcagca gcctcttcct caacgaccag gttacccttc tcaagtatgg cgtgcacgag
      901 gccatcttcg ccatgctggc ctctatcgtc aacaaggacg ggctgctggt agccaacggc
      961 agtggctttg tcacccgtga gttcctgcgc agcctccgca aacccttcag tgatatcatt
     1021 gagcctaagt ttgaatttgc tgtcaagttc aacgccctgg aacttgatga cagtgacctg
     1081 gccctattca ttgcggccat cattctgtgt ggaggtgagt gcccaacttt cttgtacaaa
     1141 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1201 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgactcta tgccaaacta ccaatacat
//
