LOCUS       sequence-285468-        3179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2975
FEATURES             Location/Qualifiers
     source          1..3179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      649..658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcatcggaa aaaggtggat aaccgaatcc ggattctcat tgagaatgga
       61 gtagctgagc ggcaaagatc tctctttgtt gtagttgggg atcgaggaaa agatcaggtg
      121 gtaatacttc atcacatgtt atccaaagca actgtgaagg ctcggccttc agtgctgtgg
      181 tgttataaga aagagctggg gtttagcagt caccggaaga aaagaatgcg acagctgcag
      241 aagaaaataa agaatggaac actgaacata aagcaggacg acccctttga actcttcata
      301 gcagccacaa acattcgcta ctgctactac aacgagaccc acaagatcct gggcaatacc
      361 ttcggcatgt gtgtgctgca ggattttgaa gccttaactc caaacttgct ggccaggact
      421 gtagaaacag tggaaggtgg tgggctagtg gtcatcctcc tacggaccat gaactcactc
      481 aagcaattgt acacagtgac tatggatgtg cattccaggt acagaactga ggcccatcag
      541 gatgtggtgg gaagatttaa tgaaaggttt attctgtctc tggcctcttg taagaagtgt
      601 ctcgtcattg atgaccagct caacatcctg cccatctcct cccacgttgc caccatggag
      661 gccctgcctc cccagactcc ggatgagagt cttggtcctt ctgatctgga gctgagggag
      721 ttgaaggaga gcttgcagga cacccagcct gtgggtgtgt tggtggactg ctgtaagact
      781 ctagaccagg ccaaagctgt cttgaaattt atcgagggca tctctgaaaa gaccctgagg
      841 agtactgttg cactcacagc tgctcgagga cggggaaaat ctgcagccct gggattggcg
      901 attgctgggg cggtggcatt tgggtactcc aatatctttg ttacctcccc aagccctgat
      961 aacctccata ctctgtttga atttgtattt aaaggatttg atgctctgca atatcaggaa
     1021 catctggatt atgagattat ccagtctcta aatcctgaat ttaacaaagc agtgatcaga
     1081 gtgaatgtat ttcgagaaca caggcagact attcagtata tacatcctgc agatgctgtg
     1141 aagctgggcc aggctgaact agttgtgatt gatgaagctg ccgccatccc cctccccttg
     1201 gtgaagagcc tacttggccc ctaccttgtt ttcatggcat ccaccatcaa tggctatgag
     1261 ggcactggcc ggtcactgtc cctcaagcta attcagcagc tccgtcaaca gagcgcccag
     1321 agccaggtca gcaccactgc tgagaataag accacgacga cagccagatt ggcatcagcg
     1381 cggacactgt atgaggtttc cctccaggag tcaatccgat acgcccctgg ggatgcagtg
     1441 gagaagtggc tgaatgactt gctgtgcctg gattgcctca acatcactcg gatagtctca
     1501 ggctgcccct tgcctgaagc ttgtgaactg tactatgtta atagagatac cctcttttgc
     1561 taccacaagg cctctgaagt tttcctccaa cggcttatgg ccctctacgt ggcttctcac
     1621 tacaagaact ctcccaatga tctccagatg ctctccgatg cacctgctca ccatctcttc
     1681 tgccttctgc ctcccgtgcc ccccacccag aatgcccttc cagaagtgct tgctgttatc
     1741 caggtgtgcc ttgaagggga gatttctcgc cagtccatct tgaacagtct gtctcgaggc
     1801 aagaaggctt caggggacct gattccatgg acagtgtcag aacagttcca agatccagac
     1861 tttggtggtc tgtctggtgg aagggtcgtt cgcattgctg ttcacccaga ttatcaaggg
     1921 atgggctatg gcagccgtgc tctgcagctg ctgcagatgt actatgaagg caggtttcct
     1981 tgtctggagg aaaaggtcct tgagacacca caggaaattc acaccgtaag cagcgaggct
     2041 gtcagcttgt tggaagaggt catcactccc cggaaggacc tgcctccttt actcctcaaa
     2101 ttgaatgaga ggcctgccga acgcctggat tacctgggtg tttcctatgg cttgaccccc
     2161 aggctcctca agttctggaa acgagctgga tttgttcctg tttatctgag acagaccccg
     2221 aatgacctga ccggagagca ctcgtgcatc atgctgaaga cgctcactga tgaggatgag
     2281 gctgaccagg gaggctggct tgcagccttc tggaaagatt tccgacggcg gttcctagcc
     2341 ttgctctcct accagttcag taccttctct ccttccctgg ctctgaacat cattcagaac
     2401 aggaacatgg ggaagccagc ccagcctgcc ctgagccggg aggagctgga agcactcttc
     2461 ctcccctatg acctgaagcg gctggagatg tattcacgga atatggtgga ctatcacctc
     2521 atcatggaca tgatcccggc catctctcgc atctatttcc tgaaccagct gggggacctg
     2581 gccctgtctg cggctcagtc ggctcttctc ttggggattg gcctgcagca taagtctgtg
     2641 gaccagctgg aaaaggagat tgagctgccc tcgggccagt tgatgggact tttcaaccgg
     2701 atcatccgca aagttgtgaa gctatttaat gaagttcagg aaaaggccat tgaggagcag
     2761 atggtggcag cgaaggatgt ggtcatggag cccacgatga agaccctcag tgacgaccta
     2821 gatgaagcag caaaggaatt tcaggagaaa cacaagaagg aagtagggaa gctgaagagc
     2881 atggacctct ctgaatacat aatccgtggg gacgatgaag agtggaatga agttttgaac
     2941 aaagctgggc cgaacgcctc gatcatcagc ctgaaaagtg acaagaaaag gaagttagag
     3001 gccaaacaag aacccaaaca gagcaagaag ttgaagaaca gagagacaaa gaacaaaaaa
     3061 gatatgaaac tgaagcggaa gaaatagtaa tgaactagtc cgtaacttga aagtatttcg
     3121 atttcttggc tttatatatc ttgtggaaag gacgagcacg aatcgtcgtt atcgtatgg
//
