LOCUS       sequence-285471-        1766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2976
FEATURES             Location/Qualifiers
     source          1..1766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1598..1622)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1630..1671
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctcaggataa tgacacattg
       61 atgagagaca tcctggggca tgagctcgct gctatgaggc tgcagaagct ggaacagcag
      121 cggcggctgt ttgaaaagaa gcagcgacag aagcgccagg agctcctcat ggttcaggcc
      181 aatcctgacg cttccccgtg gctttggcgc tcttgtctgc gggaggagcg ccttttaggt
      241 gacagaggcc ttgggaaccc tttcctccgg aagaaagtgt cagaggcaca tctgccctct
      301 ggcatccaca gtgccctggg caccgtgagc tgtggtggag acggcagggg cgagcgcggc
      361 ctcccgacac cgcggacaga agcagtgttc aggaatctcg gtctccagtc ccctttctta
      421 tcctggctcc cagacaattc cgatgcagaa ttggaggaag tctccgtgga gaatggttcc
      481 gtctctcccc caccttttaa acagtctccg agaatccgac gcaagggttg gcaagcccac
      541 caacgacctg ggacccgtgc agagggtgag agtgactccc aggatatggg agatgcacac
      601 aagtcaccca atatgggacc aaaccctgga atggatggtg actgtgtata tgaaaacttg
      661 gccttccaaa aggaagaaga cttggaaaag aagagagagg cctctgagtc tacagggacg
      721 aactcctcag cagcacacaa cgaagagttg tccaaggccc tgaaaggcga gggtggcacg
      781 gacagcgacc atatgaggca cgaagcctcc ttggcaatcc gctccccctg ccctgggctg
      841 gaggaggaca tggaagccta cgtgctgcgg ccagcgctcc cgggcaccat gatgcagtgc
      901 tacctcaccc gtgacaagca cggcgtggac aagggcttgt tccccctcta ctacctctac
      961 ctggagacct ctgacagcct gcagcgcttc ctcctggctg ggcgaaagag aagaaggagc
     1021 aaaacttcta attacctcat ctccctggat cctacacacc tatctcggga cggggacaat
     1081 ttcgtgggca aagtcagatc caatgtcttc agcaccaagt tcaccatctt tgacaatggg
     1141 gtgaatcctg accgggagca tttaaccagg aatactgccc ggatcagaca ggagctgggg
     1201 gctgtgtgtt atgagcccaa cgtcttagga tacctggggc ctcggaaaat gactgtgatt
     1261 ctcccaggaa ccaacagcca gaaccagcga atcaatgtcc agccactaaa tgaacaggag
     1321 tcgctactga gtcgttacca acgtggggac aaacaagggt tgcttttgtt gcacaacaaa
     1381 accccgtcgt gggacaagga gaacggtgtc tacacgctca atttccatgg tcgagtcact
     1441 cgggcttcgg tgaagaactt ccaaatcgtg gatcccaaac accaagaaca tctggtgctc
     1501 cagttcggcc gagtgggccc agacacattc accatggact tctgctttcc atttagcccg
     1561 ctccaggcct tcagcatctg cttgtccagt ttcaattacc caactttctt gtacaaagtg
     1621 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1681 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1741 gagctgttcg agcgccctac aaccat
//
