LOCUS       sequence-285474-        2327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2977
FEATURES             Location/Qualifiers
     source          1..2327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2159..2183)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2191..2232
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgaccacgtt gaaggaggca
       61 gtgaccttca aggacgtggc tgtggtcttc actgaggaag agctgaggct gctggacctt
      121 gcccagagga aactgtaccg agaagtgatg ctggagaact tcaggaacct gctctcagtg
      181 gggcatcaat cactccacag agatactttc cacttcctaa aggaagaaaa gttttggatg
      241 atggagacag caacccaaag agaagggaat ttaggaggca agatccaaat ggagatggag
      301 actgtttcag aatcaggaac acatgaaggc ttgttcagtc atcaaacctg ggaacaaatt
      361 tcaagtgact taaccaggtt tcaagactcc atggtaaaca gctttcagtt ctccaaacaa
      421 gatgatatgc cctgccaggt tgatgcagga ctatctataa ttcacgtaag acagaaacct
      481 tctgagggta ggacgtgtaa aaagtccttt agtgatgtct ccgtccttga tcttcatcaa
      541 caactacagt caagagagaa gtctcataca tgtgatgaat gtggaaagag tttctgttat
      601 agctcagctc ttcgtattca tcagagagtt cacatggggg agaaactcta taattgtgat
      661 gtgtgtggta aggaattcaa tcagagctca catctgcaaa ttcatcagag aatccacact
      721 ggagagaaac cattcaaatg tgagcagtgt gggaaaggct ttagtcgtag atcaggactt
      781 tatgttcatc gtaaattaca cacaggagtg aaacctcata tttgtgagaa atgtgggaag
      841 gccttcattc atgattccca gcttcaggaa catcaaagaa tccatactgg ggagaagcca
      901 ttcaaatgtg atatatgttg taagagcttc cgtagtagag caaatcttaa taggcattcc
      961 atggttcaca tgcgagagaa accattcaga tgtgatacat gtggtaagag ctttggtctg
     1021 aaatcagcac ttaatagtca tcgcatggtc cacacaggag agaaacggta caaatgtgag
     1081 gaatgtggaa aacgcttcat ttataggcaa gatctttata agcatcaaat agaccacaca
     1141 ggggagaagc catataattg taaagaatgt ggaaagagct tcagatgggc ctcaggtctt
     1201 tcaagacatg tgcgagtcca cagtggagag acaacattca aatgtgaaga atgtgggaag
     1261 ggattttata caaattcaca acgttattct caccagagag cgcacagtgg agaaaagcca
     1321 tatagatgtg aggagtgtgg gaagggctac aaaaggaggt tggatcttga ctttcatcag
     1381 agggtccaca gaggagagaa accctataat tgtaaggaat gtgggaagag ctttggctgg
     1441 gcctcgtgtc ttttgaatca tcagagaatc cacagtggag aaaaaccatt taaatgtgaa
     1501 gaatgtggga aaagatttac tcagaattca caactttata cccatcgtag agtccacagt
     1561 ggagaaaaac cattcaaatg tgaagagtgt gggaaaagat ttactcagaa ttcacaactt
     1621 tattctcatc gcagagtcca cactggagta aagccataca aatgtgaaga gtgtgggaag
     1681 ggcttcaaca gtaagtttaa tcttgacatg caccagaggg tccacaccgg agagagacct
     1741 tataattgta aagaatgtgg gaagagcttt agccgggcct caagtatttt gaatcataag
     1801 agactccatg gtgatgaaaa gccattcaaa tgtgaagagt gtgggaagag atttactgag
     1861 aattcacagc ttcattccca tcagagggtt cacactgggg aaaagccata caaatgtgag
     1921 aagtgtggaa agagcttcag atgggcctca actcatctaa cccatcagag actccacagt
     1981 agagaaaaac tacttcaatg tgaggactgt gggaagagca ttgtgcacag ttcatgcctt
     2041 aaagaccaac aaagagacca aagtggagag aaaacatcta aatgtgagga ctgtgggaag
     2101 cgctacaaga ggcgcttgaa tcttgatacg cttttgtcat tatttttaaa tgacacattg
     2161 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2221 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2281 tatatatctt gtggaaagga cgaagaccac tgatcaagat ctattta
//
