LOCUS       sequence-285477-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2978
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(809..833)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             841..882
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcaccagcca accaggggcc
       61 tgcccatgcc agggagctgc aagccgcccc gccattctct acgcacttct gagctccagc
      121 ctcaaggctg tcccccgacc ccgtagccgc tgcctatgta ggcagcaccg gcccgtccag
      181 ctatgtgcac ctcatcgcac ctgccgggag gccttggatg ttctggccaa gacagtggcc
      241 ttcctcagga acctgccatc cttctggcag ctgcctcccc aggaccagcg gcggctgctg
      301 cagggttgct ggggccccct cttcctgctt gggttggccc aagatgctgt gacctttgag
      361 gtggctgagg ccccggtgcc cagcatactc aagaagattc tgctggagga gcccagcagc
      421 agtggaggca gtggccaact gccagacaga ccccagccct ccctggctgc ggtgcagtgg
      481 cttcaatgct gtctggagtc cttctggagc ctggagctta gccccaagga atatgcctgc
      541 ctgaaaggga ccatcctctt caaccccgat gtgccaggcc tccaagccgc ctcccacatt
      601 gggcacctgc agcaggaggc tcactgggtg ctgtgtgaag tcctggaacc ctggtgccca
      661 gcagcccaag gccgcctgac ccgtgtcctc ctcacggcct ccaccctcaa gtccattccg
      721 accagcctgc ttggggacct cttctttcgc cctatcattg gagatgttga catcgctggc
      781 cttcttgggg acatgctttt gctcaggtgc ccaactttct tgtacaaagt ggttgatatc
      841 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      901 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattcactc
      961 tattaaattc ctatctt
//
