LOCUS       sequence-28548-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable suppression of miR-31 function.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC,
            Brock JE, Richardson AL, Weinberg RA
  TITLE     A pleiotropically acting microRNA, miR-31, inhibits breast cancer 
            metastasis.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1032-46.
  PUBMED    19524507
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1032-46."
COMMENT     Sequence Label: pBABE-puro-mIR-31 sponge
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(356..373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(385..405)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        390..719
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      570..705
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(729..748)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ctcggctgga cgagctgtac aagaagctta gccatggctt cccgccggag gtggaggagc
       61 aggatgatgg cacgctgccc atgtcttgtg cccaggagag cgggatggac cgtcaccctg
      121 cagcctgtgc ttctgctagg atcaatgtgt aggcggccgc ctcgagagct atgcctcgtc
      181 ttgcctccgg agctatgcct cgtcttgcct ccggagctat gcctcgtctt gcctccggag
      241 ctatgcctcg tcttgcctcc ggagctatgc ctcgtcttgc ctccggagct atgcctcgtc
      301 ttgcctccgg agctatgcct cgtcttgcct gggcccgttt aaacccgctg atcagcctcg
      361 actgtgcctt ctagtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      421 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcan
      541 naaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      601 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      661 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctangc ttttgcaaaa
      721 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
      781 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
//
