LOCUS       sequence-285480-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2979
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(815..839)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             847..888
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgagcaccag ccaaccaggg
       61 gcctgcccat gccagggagc tgcaagccgc cccgccattc tctacgcact tctgagctcc
      121 agcctcaagg ctgtcccccg accccgtagc cgctgcctat gtaggcagca ccggcccgtc
      181 cagctatgtg cacctcatcg cacctgccgg gaggccttgg atgttctggc caagacagtg
      241 gccttcctca ggaacctgcc atccttctgg cagctgcctc cccaggacca gcggcggctg
      301 ctgcagggtt gctggggccc cctcttcctg cttgggttgg cccaagatgc tgtgaccttt
      361 gaggtggctg aggccccggt gcccagcata ctcaagaaga ttctgctgga ggagcccagc
      421 agcagtggag gcagtggcca actgccagac agaccccagc cctccctggc tgcggtgcag
      481 tggcttcaat gctgtctgga gtccttctgg agcctggagc ttagccccaa ggaatatgcc
      541 tgcctgaaag ggaccatcct cttcaacccc gatgtgccag gcctccaagc cgcctcccac
      601 attgggcacc tgcagcagga ggctcactgg gtgctgtgtg aagtcctgga accctggtgc
      661 ccagcagccc aaggccgcct gacccgtgtc ctcctcacgg cctccaccct caagtccatt
      721 ccgaccagcc tgcttgggga cctcttcttt cgccctatca ttggagatgt tgacatcgct
      781 ggccttcttg gggacatgct tttgctcagg tagaacccag ctttcttgta caaagtggtt
      841 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
      901 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
      961 agttcgacaa cctctatgtc gat
//
