LOCUS       sequence-285486-        1673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2981
FEATURES             Location/Qualifiers
     source          1..1673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1505..1529)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1537..1578
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga aagagttgga tccaaagaat
       61 gacatttcgg aagacaagct ctccgttgtt ggggaggcca cggggggacc cacgaggaat
      121 ggtgccaggg gtcctggctc agaaggagtg tgggaaccag gcagctggcc agagaggccg
      181 cggggagatg caggtgcaga gtgggagcca ttgggaattc cccaggggaa caaactctta
      241 gggggctcag tacccgcatg tcatgaactg aaggcatttg ccaaccaagg ctgtgtcctg
      301 gtcccaccac ggctggacga ccccacagaa aagggggcct gtccacccgt aaggcgtggc
      361 aagaacttct ccagcacttc agacctcagt aagcccccca tgccctgcga ggagaagaaa
      421 acctacgact gcagcgagtg tggcaaggcc tttagccgaa gctcgtccct gataaagcac
      481 caaaggatcc acacgggaga aaagccgttt gagtgtgaca cctgtgggaa gcacttcatc
      541 gagcgctcgt ccctcaccat ccaccagcgg gtgcacacgg gcgagaagcc ctatgcctgc
      601 ggggactgcg gcaaggcctt cagccagcgc atgaacctca ctgtgcacca gcgcacgcac
      661 acgggcgaga agccgtatgt gtgcgacgtg tgtggcaagg ccttccggaa gacttcctct
      721 ctcacccagc acgagcggat ccacacgggg gagaagccct acgcgtgcgg ggactgcggc
      781 aaggccttca gccagaacat gcacctcatc gtgcaccagc gcacgcacac cggggagaag
      841 ccgtacgtgt gccccgagtg cgggcgagcc ttcagccaga acatgcacct gaccgagcac
      901 cagcgcacgc acaccgggga gaagccgtac gcctgcaagg agtgcggcaa ggccttcaac
      961 aagagctcct ctctcaccct gcaccagagg aaccacaccg gcgagaagcc ctacgtgtgc
     1021 ggcgagtgcg gcaaggcctt cagccagagc tcctacctca tccagcacca gcgcttccac
     1081 atcggcgtga agccgttcga gtgcagcgag tgcggcaagg ccttcagcaa gaactcctcg
     1141 ctcacgcagc accagcgcat ccacaccggc gagaagccct acgagtgcta catctgcaag
     1201 aagcacttca cggggcgctc gtccctcatc gtgcaccaga tcgtgcacac cggggagaag
     1261 ccctacgtgt gcggcgagtg cggcaaggcc ttcagccaga gcgcctacct catcgagcac
     1321 cagcggatcc acaccggcga gaagccgtac aggtgcggcc agtgcgggaa gtccttcatc
     1381 aagaactcct ccctcactgt gcaccagcgg atccacacgg gcgagaagcc ctaccgatgc
     1441 ggcgagtgcg ggaagacctt cagccgcaac acgaacctga cgcgccacct gcggattcac
     1501 acctgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     1561 ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1621 tggctttata tatcttgtgg aaaggacgat agtcccgggc ccatatgcat acc
//
