LOCUS       sequence-285492-        2423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2983
FEATURES             Location/Qualifiers
     source          1..2423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2255..2279)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2287..2328
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cgcctcagaa gcacggcggt
       61 gggggagggg gcggctcggg gcccagcgcg gggtccgggg gaggcggctt cgggggttcg
      121 gcggcggtgg cggcggcgac ggcttcgggc ggcaaatccg gcggcgggag ctgtggaggg
      181 ggtggcagtt actcggcctc ctcctcctcc tccgcggcgg cagcggcggg ggctgcggtg
      241 ttaccggtga agaagccgaa aatggagcac gtccaggctg accacgagct tttcctccag
      301 gcctttgaga agccaacaca gatctataga tttcttcgaa ctcggaatct catagcacca
      361 atatttttgc acagaactct tacttacatg tctcatcgaa actccagaac aaacatcaaa
      421 aggaaaacat ttaaagttga tgatatgtta tcaaaagtag agaaaatgaa aggagagcaa
      481 gaatctcata gcttgtcagc tcatttgcag cttacgttta ctggtttctt ccacaaaaat
      541 gataagccat caccaaactc agaaaatgaa caaaattctg ttaccctgga agtcctgctt
      601 gtgaaagttt gccacaaaaa aagaaaggat gtaagttgtc caataaggca agttcccaca
      661 ggtaaaaagc aggtgccttt gaatcctgac ctcaatcaaa caaaacccgg aaatttcccg
      721 tcccttgcag tttccagtaa tgaatttgaa cctagtaaca gccatatggt gaagtcttac
      781 tcgttgctat ttagagtgac tcgtccagga agaagagagt ttaatggaat gattaatgga
      841 gaaaccaatg aaaatattga tgtcaatgaa gagcttccag ccagaagaaa acgaaatcgt
      901 gaggatgggg aaaagacatt tgttgcacaa atgacagtat ttgataaaaa caggcgctta
      961 cagcttttag atggggaata tgaagtagcc atgcaggaaa tggaagaatg tccaataagc
     1021 aagaaaagag caacatggga gactattctt gatgggaaga ggctgcctcc attcgaaaca
     1081 ttttctcagg gacctacgtt gcagttcact cttcgttgga caggagagac caatgataaa
     1141 tctacggctc ctattgccaa acctcttgcc actagaaatt cagagagtct ccatcaggaa
     1201 aacaagcctg gttcagttaa acctactcaa actattgctg ttaaagaatc attgactaca
     1261 gatctacaaa caagaaaaga aaaggatact ccaaatgaaa accgacaaaa attaagaata
     1321 ttttatcagt ttctctataa caacaataca aggcaacaaa ctgaagcaag agatgacctg
     1381 cattgccctt ggtgtactct gaactgccgc aaactttata gtttactcaa gcatcttaaa
     1441 ctctgccata gcagatttat cttcaactat gtttatcatc caaaaggtgc taggatagat
     1501 gtttctatca atgagtgtta tgatggctcc tatgcaggaa atcctcagga tattcatcgc
     1561 caacctggat ttgcttttag tcgcaacgga ccagttaaga gaacacctat cacacatatt
     1621 cttgtgtgca ggccaaaacg aacaaaagca agcatgtctg aatttcttga atctgaagat
     1681 ggggaagtag aacagcaaag aacatatagt agtggccaca atcgtctgta tttccatagt
     1741 gatacctgct tacctctccg tccacaagaa atggaagtag atagtgaaga tgaaaaggat
     1801 cctgaatggc taagagaaaa aaccattaca caaattgaag agttttctga tgttaatgaa
     1861 ggagagaaag aagtgatgaa actctggaat ctccatgtca tgaagcatgg gtttattgct
     1921 gacaatcaaa tgaatcatgc ctgtatgctg tttgtagaaa attatggaca gaaaataatt
     1981 aagaagaatt tatgtcgaaa cttcatgctt catctagtca gcatgcatga ctttaatctt
     2041 attagcataa tgtcaataga taaagctgtt accaagctcc gtgaaatgca gcaaaaatta
     2101 gaaaaggggg aatctgcttc ccctgcaaac gaagaaataa ctgaagaaca aaatgggaca
     2161 gcaaatggat ttagtgaaat taactcaaaa gagaaagctt tggaaacaga tagtgtctca
     2221 ggggtttcaa aacagagcaa aaaacaaaaa ctctgcccaa ctttcttgta caaagtggtt
     2281 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     2341 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2401 ttaacttcat accccgtatc ctt
//
