LOCUS       sequence-285495-        1685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2984
FEATURES             Location/Qualifiers
     source          1..1685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(858..869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1517..1541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1549..1590
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgtctacagc ctctgccgcc
       61 tcctcctcct cctcgtcttc ggccggtgag atgatcgaag ccccttccca ggtcctcaac
      121 tttgaagaga tcgactacaa ggagatcgag gtggaagagg ttgttggaag aggagccttt
      181 ggagttgttt gcaaagctaa gtggagagca aaagatgttg ctattaaaca aatagaaagt
      241 gaatctgaga ggaaagcgtt tattgtagag cttcggcagt tatcccgtgt gaaccatcct
      301 aatattgtaa agctttatgg agcctgcttg aatccagtgt gtcttgtgat ggaatatgct
      361 gaagggggct ctttatataa tgtgctgcat ggtgctgaac cattgccata ttatactgct
      421 gcccacgcaa tgagttggtg tttacagtgt tcccaaggag tggcttatct tcacagcatg
      481 caacccaaag cgctaattca cagggacctg aaaccaccaa acttactgct ggttgcaggg
      541 gggacagttc taaaaatttg tgattttggt acagcctgtg acattcagac acacatgacc
      601 aataacaagg ggagtgctgc ttggatggca cctgaagttt ttgaaggtag taattacagt
      661 gaaaaatgtg acgtcttcag ctggggtatt attctttggg aagtgataac gcgtcggaaa
      721 ccctttgatg agattggtgg cccagctttc cgaatcatgt gggctgttca taatggtact
      781 cgaccaccac tgataaaaaa tttacctaag cccattgaga gcctgatgac tcgttgttgg
      841 tctaaagatc cttcccagcg cccttcaatg gaggaaattg tgaaaataat gactcacttg
      901 atgcggtact ttccaggagc agatgagcca ttacagtatc cttgtcagta ttcagatgaa
      961 ggacagagca actctgccac cagtacaggc tcattcatgg acattgcttc tacaaatacg
     1021 agtaacaaaa gtgacactaa tatggagcaa gttcctgcca caaatgatac tattaagcgc
     1081 ttagaatcaa aattgttgaa aaatcaggca aagcaacaga gtgaatctgg acgtttaagc
     1141 ttgggagcct cccgtgggag cagtgtggag agcttgcccc caacctctga gggcaagagg
     1201 atgagtgctg acatgtctga aatagaagct aggatcgccg caaccacagg caacggacag
     1261 ccaagacgta gatccatcca agacttgact gtaactggaa cagaacctgg tcaggtgagc
     1321 agtaggtcat ccagtcccag tgtcagaatg attactacct caggaccaac ctcagaaaag
     1381 ccaactcgaa gtcatccatg gacccctgat gattccacag ataccaatgg atcagataac
     1441 tccatcccaa tggcttatct tacactggat caccaactac aggcaagaac tagttgcaga
     1501 actggaccag gatagaaccc agctttcttg tacaaagtgg ttgatatcgg taagcctatc
     1561 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1621 atttcgattt cttggcttta tatatcttgt ggaaaggacg agtcggacgc ggtctctgat
     1681 gaccg
//
