LOCUS       sequence-285498-        1547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2985
FEATURES             Location/Qualifiers
     source          1..1547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1379..1403)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1411..1452
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gggaaccccg ggctggggcc
       61 gccctggacg atggcagcgg ctggacgggc agtgaggaag gcagtgagga gggtaccggc
      121 ggcagtgagg gggctggggg tgacgggggc ccggatgcag agggggtgtg gagcccagac
      181 attgagcaga gcttccagga ggccctggcc atctatccac cctgcggccg ccggaaaata
      241 attttgtctg atgaaggcaa gatgtatggt cggaatgaac tgatcgcccg ctacatcaag
      301 ctgagaacgg ggaagacccg aactcgaaaa caggtttcta gtcacatcca ggttttggcc
      361 cgaaggaaat caagggaaat ccagtccaag ttgaaggacc aggtttccaa ggacaaggct
      421 ttccagacaa tggcaaccat gtcctctgcc cagctcatct ccgcgccttc tctgcaggcc
      481 aaactgggtc ccactggtcc tcaggcctct gagcttttcc agttttggtc tggaggatct
      541 gggcccccct ggaatgttcc agatgtgaag ccattctcac agacaccgtt caccttgtca
      601 ctgactcccc catctactga cctcccaggg tacgagcccc cccaagccct ctcacccctg
      661 cccccaccta ccccatcgcc cccagcctgg caggctcggg gcctgggcac cgcccggttg
      721 cagctggtag agttctcagc cttcgtggaa ccgccagatg cagttgattc ttaccagagg
      781 cacctgttcg tgcacatcag ccagcactgc cccagccccg gagcgccgcc gctcgagagt
      841 gtggacgtcc ggcagatcta cgacaaattc cctgagaaaa agggtggcct ccgagagcta
      901 tatgatcgtg gcccccccca tgccttcttc ctggtcaagt tctgggcgga cctgaactgg
      961 ggcccaagtg gtgaggaggc aggggccggt ggcagcatca gcagtggtgg cttctacgga
     1021 gtgagcagcc agtatgagag cctggaacac atgaccctca cctgttcctc caaggtctgc
     1081 tcttttggca agcaggtggt ggagaaggtg gagacggaac gggcccagct ggaggacggc
     1141 agatttgtgt accgcctgct gcgctcgccc atgtgcgagt acctggtgaa tttcttgcac
     1201 aagttgcggc agctgcctga gcgatacatg atgaacagcg tcctggaaaa cttcaccatc
     1261 ctccaggtgg tgacaaacag agacacccag gaactgctgc tctgcaccgc ctatgtcttc
     1321 gaggtctcca ccagcgagcg tggggcccag catcacattt accgcctggt cagggactgc
     1381 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     1441 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1501 tatatatctt gtggaaagga cgaggacgtc cgaaggagac catattc
//
