LOCUS       sequence-285501-        1664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2986
FEATURES             Location/Qualifiers
     source          1..1664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1132..1152
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(1496..1520)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1528..1569
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tagctgggat gttagggctc
       61 agggaagaaa agtcagaaga ccaggacctc cagggcctca aggacaaacc cctcaagttt
      121 aaaaaggtga agaaagataa gaaagaagag aaagagggca agcatgagcc cgtgcagcca
      181 tcagcccacc actctgctga gcccgcagag gcaggcaaag cagagacatc agaagggtca
      241 ggctccgccc cggctgtgcc ggaagcttct gcctccccca aacagcggcg ctccatcatc
      301 cgtgaccggg gacccatgta tgatgacccc accctgcctg aaggctggac acggaagctt
      361 aagcaaagga aatctggccg ctctgctggg aagtatgatg tgtatttgat caatccccag
      421 ggaaaagcct ttcgctctaa agtggagttg attgcgtact tcgaaaaggt aggcgacaca
      481 tccctggacc ctaatgattt tgacttcacg gtaactggga gagggagccc ctcccggcga
      541 gagcagaaac cacctaagaa gcccaaatct cccaaagctc caggaactgg cagaggccgg
      601 ggacgcccca aagggagcgg caccacgaga cccaaggcgg ccacgtcaga gggtgtgcag
      661 gtgaaaaggg tcctggagaa aagtcctggg aagctccttg tcaagatgcc ttttcaaact
      721 tcgccagggg gcaaggctga ggggggtggg gccaccacat ccacccaggt catggtgatc
      781 aaacgccccg gcaggaagcg aaaagctgag gccgaccctc aggccattcc caagaaacgg
      841 ggccgaaagc cggggagtgt ggtggcagcc gctgccgccg aggccaaaaa gaaagccgtg
      901 aaggagtctt ctatccgatc tgtgcaggag accgtactcc ccatcaagaa gcgcaagacc
      961 cgggagacgg tcagcatcga ggtcaaggaa gtggtgaagc ccctgctggt gtccaccctc
     1021 ggtgagaaga gcgggaaagg actgaagacc tgtaagagcc ctgggcggaa aagcaaggag
     1081 agcagcccca aggggcgcag cagcagcgcc tcctcacccc ccaagaagga gcaccaccac
     1141 catcaccacc actcagagtc cccaaaggcc cccgtgccac tgctcccacc cctgccccca
     1201 cctccacctg agcccgagag ctccgaggac cccaccagcc cccctgagcc ccaggacttg
     1261 agcagcagcg tctgcaaaga ggagaagatg cccagaggag gctcactgga gagcgacggc
     1321 tgccccaagg agccagctaa gactcagccc gcggttgcca ccgccgccac ggccgcagaa
     1381 aagtacaaac accgagggga gggagagcgc aaagacattg tttcatcctc catgccaagg
     1441 ccaaacagag aggagcctgt ggacagccgg acgcccgtga ccgagagagt tagctgccca
     1501 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1561 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1621 atatcttgtg gaaaggacga tcggacatat cggtagccct taac
//
