LOCUS       sequence-285504-        1781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2987
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1613..1637)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1645..1686
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggagtcagc tccggcagcc
       61 cccgaccccg ccgccagcga gccaggcagc agcggcgcgg acgcggccgc cggctccagg
      121 gagaccccgc tgaaccagga atccgcccgc aagagcgagc cgcctgcccc ggtgcgcaga
      181 cagagctatt ccagcaccag cagaggtatc tcagtaacga agaagacaca tacatctcaa
      241 attgaaatta ttccatgcaa gatctgtgga gacaaatcat caggaatcca ttatggtgtc
      301 attacatgtg aaggctgcaa gggctttttc aggagaagtc agcaaagcaa tgccacctac
      361 tcctgtcctc gtcagaagaa ctgtttgatt gatcgaacca gtagaaaccg ctgccaacac
      421 tgtcgattac agaaatgcct tgccgtaggg atgtctcgag atgctgtaaa atttggccga
      481 atgtcaaaaa agcagagaga cagcttgtat gcagaagtac agaaacaccg gatgcagcag
      541 cagcagcgcg accaccagca gcagcctgga gaggctgagc cgctgacgcc cacctacaac
      601 atctcggcca acgggctgac ggaacttcac gacgacctca gtaactacat tgacgggcac
      661 acccctgagg ggagtaaggc agactccgcc gtcagcagct tctacctgga catacagcct
      721 tccccagacc agtcaggtct tgatatcaat ggaatcaaac cagaaccaat atgtgactac
      781 acaccagcat caggcttctt tccctactgt tcgttcacca acggcgagac ttccccaact
      841 gtgtccatgg cagaattaga acaccttgca cagaatatat ctaaatcgca tctggaaacc
      901 tgccaatact tgagagaaga gctccagcag ataacgtggc agaccttttt acaggaagaa
      961 attgagaact atcaaaacaa gcagcgggag gtgatgtggc aattgtgtgc catcaaaatt
     1021 acagaagcta tacagtatgt ggtggagttt gccaaacgca ttgatggatt tatggaactg
     1081 tgtcaaaatg atcaaattgt gcttctaaaa gcaggttctc tagaggtggt gtttatcaga
     1141 atgtgccgtg cctttgactc tcagaacaac accgtgtact ttgatgggaa gtatgccagc
     1201 cccgacgtct tcaaatcctt aggttgtgaa gactttatta gctttgtgtt tgaatttgga
     1261 aagagtttat gttctatgca cctgactgaa gatgaaattg cattattttc tgcatttgta
     1321 ctgatgtcag cagatcgctc atggctgcaa gaaaaggtaa aaattgaaaa actgcaacag
     1381 aaaattcagc tagctcttca acacgtccta cagaagaatc accgagaaga tggaatacta
     1441 acaaagttaa tatgcaaggt gtctacatta agagccttat gtggacgaca tacagaaaag
     1501 ctaatggcat ttaaagcaat atacccagac attgtgcgac ttcattttcc tccattatac
     1561 aaggagttgt tcacttcaga atttgagcca gcaatgcaaa ttgatgggta agacccagct
     1621 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1681 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgaccg gaaaggcaaa tattagttta t
//
