LOCUS       sequence-285513-        2432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2990
FEATURES             Location/Qualifiers
     source          1..2432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2264..2288)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2296..2337
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc tttctctgaa gaaatactta
       61 acggaaggac ttctccagtt caccattctg ctgagtttga ttggggtacg ggtggacgtg
      121 gatacttacc tgacctcaca gcttccccca ctccgggaga tcatcctggg gcccagttct
      181 gcctatactc agacccagtt ccacaacctg aggaatacct tggatggcta tggtatccac
      241 cccaagagca tagacctgga caattacttc actgcccggc ggctcctcag tcaggtgagg
      301 gccctggaca ggttccaggt gccaaccact gaggtaaatg cctggctggt tcaccgagac
      361 ccagaggggt ctgtctctgg cagtcagccc aactcaggcc tcgccctcga gagttccagt
      421 ggcctccaag atgtgacagg cccagacaac ggggtgcgag aaagcgaaac ggagcaggga
      481 ttcggtgaag atttggagga tttgggggct gtagcccccc cagtcagtgg agacttaacc
      541 aaagaggaca tagatctgat tgacatcctt tggcgacagg atattgatct gggggctggg
      601 cgtgaggttt ttgactatag tcaccgccag aaggagcagg atgtggagaa ggagctgcga
      661 gatggaggcg agcaggacac ctgggcaggc gagggcgcgg aagctctggc acggaacctg
      721 ctagtggatg gagagactgg ggagagcttc cctgcacagt ttccagcaga catttccagc
      781 ataacagaag cagtgcctag tgagagtgag ccccctgctc ttcaaaacaa cctcttgtct
      841 cctcttctga ccgggacaga gtcaccattt gatttggaac agcagtggca agatctcatg
      901 tccatcatgg aaatgcaggc catggaagtg aacacatcag caagtgaaat cctgtacagt
      961 gcccctcctg gagacccact gagcaccaac tacagccttg cccccaacac tcccatcaat
     1021 cagaatgtca gcctgcatca ggcgtccctg gggggctgca gccaggactt cttactcttc
     1081 agccccgagg tggaaagcct gcctgtggcc agtagctcca cgctgctccc gttggccccc
     1141 agcaattcta ccagcctcaa ctccaccttc ggctccacca acctgacagg gctcttcttt
     1201 ccaccccagc tcaatggcac agccaatgac acagcaggcc cagagctgcc tgaccctttg
     1261 gggggtctgt tagatgaagc tatgttggat gagatcagcc ttatggacct ggccattgaa
     1321 gaaggcttta accctgtgca ggcctcccag ctggaggagg aatttgactc tgactcaggc
     1381 ctttccttag actcgagcca tagcccttct tccctaagca gctctgaagg cagttcttcc
     1441 tcttcttcct cctcctcttc ctcttcttcc tctgcttctt cctctgcctc ttcctccttt
     1501 tctgaggaag gtgcggttgg ctacagctct gactctgaga ccctggatct ggaagaggcc
     1561 gagggtgctg tgggctacca gcctgagtat tccaagttct gccgcatgag ctaccaggat
     1621 ccagctcagc tctcatgcct gccctacctg gagcacgtgg gccacaacca cacatacaac
     1681 atggcaccca gtgccctgga ctcagccgac ctgccaccac ccagtgccct caagaaaggc
     1741 agcaaggaga agcaggctga cttcctggac aagcagatga gccgggatga gcaccgagcc
     1801 cgagccatga agatcccttt caccaatgac aaaatcatca acctgcctgt ggaggagttc
     1861 aatgaactgc tgtccaaata ccagttgagt gaagcccagc tgagcctcat ccgagacatc
     1921 cggcgccggg gcaagaacaa gatggcggcg cagaactgcc gcaagcgcaa gctggacacc
     1981 atcctgaatc tggagcgtga tgtggaggac ctgcagcgtg acaaagcccg gctgctgcgg
     2041 gagaaagtgg agttcctgcg ctccctgcga cagatgaagc agaaggtcca gagcctgtac
     2101 caggaggtgt ttgggcggct gcgagatgag aacggacgac cctactcgcc cagtcagtat
     2161 gcgctccagt acgccgggga cggcagtgtc ctcctcatcc cccgcacgat ggccgaccag
     2221 caggcccggc ggcaggagag gaagccaaag gaccggagaa agtgcccaac tttcttgtac
     2281 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     2341 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2401 aaggacgata tggagtgccg ttttggcgtt ta
//
