LOCUS       sequence-285519-        1139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2992
FEATURES             Location/Qualifiers
     source          1..1139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(971..995)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1003..1044
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg acttaaaact aaaagactgt
       61 gaattttggt atagtctcca tggccaggtc ccagggctgc tggactggga catgaggaat
      121 gagttatttc tgccttgtac cacagaccag tgctctttag ctgagcaaat ccttgccaaa
      181 tacagagtcg gagtaatgaa gcccccagaa atgcctcaga agaggagacc cagtcctgat
      241 ggagatggtc ctccctgtga acccaatctg tggatgtggg tggaccccaa tatcctgtgc
      301 ccccttggca gccaggaggc cccaaagccc agtggaaaag aggatctgac aaacatttct
      361 cctttccctc agcccccaca aaaagacgaa gggtctaact gctcagagga caaagtggta
      421 gagtctctgc catcttcctc cagtgagcag tctcctttac agaagcaggg tatccattcc
      481 cccagtgact ttgagctcac agaagaggag gctgaggaac cagacgacaa ctccctccag
      541 tcccctgaaa tgaaatgtta ccagagccag aaactatggc aaatcaacaa ccaagagaag
      601 tcctggcaaa ggccccctct caattgtagc caccttattg ccctagcatt aagaaacaac
      661 ccccactgtg gcctcagtgt gcaggagatc tacaatttca cccgacagca tttccccttt
      721 ttctggacag ctccggatgg ctggaagagc accattcatt acaacctctg cttcctggac
      781 agctttgaga aggtgccaga cagccttaag gatgaagata atgcaagacc tcgctcttgc
      841 ctttggaagc tcactaagga ggggcaccgc cgcttttggg aggagactcg tgtcttagcc
      901 tttgctcaaa gggagagaat ccaagagtgc atgagtcagc cagagttgtt gacctctctc
      961 tttgatcttt gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
     1021 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1081 atttcttggc tttatatatc ttgtggaaag gacgaacaag tgtagacgta aatctcaat
//
