LOCUS       sequence-285522-        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2993
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1268..1292)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1300..1341
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc acaggaagca cctccaggag
       61 attccagacc tgagtagcaa cgttgccacc agcttcacgt ggggatggga ttccagcaag
      121 acttctgaac tgctgtcagg catgggggtc tccgccctgg agaaagagga gcccgacagt
      181 gagaacatcc cccaggaact gctctcaaac ctgggccacc cggagagccc cccacggaaa
      241 cggctgaaga gcaaagggag tgacaaagac tttgtgattg tccgcaggcc taagctaaat
      301 cgagagaact ttccaggtgt ttcatgggac tcccttccgg atgagctgct cttgggaatc
      361 ttttcctgtc tgtgcctccc tgagctgcta aaggtctctg gtgtttgtaa gaggtggtat
      421 cgcctagcgt ctgatgagtc tctatggcag accttagacc tcacaggtaa aaatctgcac
      481 ccggatgtga ctggtcggtt gctgtctcaa ggggtgattg ccttccgctg cccacgatca
      541 tttatggacc aaccattggc tgaacatttc agcccttttc gtgtacagca catggaccta
      601 tcgaactcag ttatagaagt gtccaccctc cacggcatac tgtctcagtg ttccaagttg
      661 cagaatctaa gcctggaagg cctgcggctt tcggatccca ttgtcaatac tctcgcaaaa
      721 aactcaaatt tagtgcgact taacctttct gggtgttctg gattctctga atttgccctg
      781 cagactttgc taagcagctg ttccagactg gatgagctga acctctcctg gtgttttgat
      841 ttcactgaaa agcatgtaca ggtggctgtt gcgcatgtgt cagagaccat cacccagctg
      901 aatcttagcg gctacagaaa gaatctccag aaatcagatc tctctacttt agttagaaga
      961 tgccccaatc ttgtccatct agacttaagt gatagtgtca tgctaaagaa tgactgcttt
     1021 caggaatttt tccagctcaa ctacctccaa cacctatcac tcagtcggtg ctatgatata
     1081 atacctgaaa ctttactatt agtgacaaga gctggggtta ggatccggtt ggactctgac
     1141 atcggatgcc ctcaaacata cagaacttcc aaactcaagt ccagccataa gctattttgc
     1201 caacatgtca gagtaatctg tatttttgta tgtgatttct acttttatag acttgtttta
     1261 aaacaatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1321 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1381 tcttggcttt atatatcttg tggaaaggac gaatctggta atgttgataa taccct
//
