LOCUS       sequence-285525-        1484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2994
FEATURES             Location/Qualifiers
     source          1..1484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1316..1340)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1348..1389
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tgcacaggaa gcacctccag
       61 gagattccag acctgagtag caacgttgcc accagcttca cgtggggatg ggattccagc
      121 aagacttctg aactgctgtc aggcatgggg gtctccgccc tggagaaaga ggagcccgac
      181 agtgagaaca tcccccagga actgctctca aacctgggcc acccggagag ccccccacgg
      241 aaacggctga agagcaaagg gagtgacaaa gactttgtga ttgtccgcag gcctaagcta
      301 aatcgagaga actttccagg tgtttcatgg gactcccttc cggatgagct gctcttggga
      361 atcttttcct gtctgtgcct ccctgagctg ctaaaggtct ctggtgtttg taagaggtgg
      421 tatcgcctag cgtctgatga gtctctatgg cagaccttag acctcacagg taaaaatctg
      481 cacccggatg tgactggtcg gttgctgtct caaggggtga ttgccttccg ctgcccacga
      541 tcatttatgg accaaccatt ggctgaacat ttcagccctt ttcgtgtaca gcacatggac
      601 ctatcgaact cagttataga agtgtccacc ctccacggca tactgtctca gtgttccaag
      661 ttgcagaatc taagcctgga aggcctgcgg ctttcggatc ccattgtcaa tactctcgca
      721 aaaaactcaa atttagtgcg acttaacctt tctgggtgtt ctggattctc tgaatttgcc
      781 ctgcagactt tgctaagcag ctgttccaga ctggatgagc tgaacctctc ctggtgtttt
      841 gatttcactg aaaagcatgt acaggtggct gttgcgcatg tgtcagagac catcacccag
      901 ctgaatctta gcggctacag aaagaatctc cagaaatcag atctctctac tttagttaga
      961 agatgcccca atcttgtcca tctagactta agtgatagtg tcatgctaaa gaatgactgc
     1021 tttcaggaat ttttccagct caactacctc caacacctat cactcagtcg gtgctatgat
     1081 ataatacctg aaactttact tgaacttgga gaaattccca cactaaaaac actacaagtt
     1141 tttggaatcg tgccagatgg tacccttcaa ctgttaaagg aagcccttcc tcatctacag
     1201 attaattgct cccatttcac caccattgcc aggccaacta ttggcaacaa aaagaaccag
     1261 gagatatggg gcatcaaatg ccgactgaca ctgcaaaagc ccagttgtct atagaaccca
     1321 gctttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     1381 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1441 atatcttgtg gaaaggacga ccgtccctat gtcactgcta ttac
//
