LOCUS       sequence-285531-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2996
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(854..878)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             886..927
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga aaggcaagaa aggtattgtt
       61 gcagcatctg gcagtgagac tgaggatgag gacagcatgg acattccctt ggacctttct
      121 tcatccgctg gctcaggcaa gagaaggaga aggggcaacc tacccaagga gtctgtgcag
      181 attcttcggg attggctgta tgagcaccgt tacaatgcct atccttcaga gcaagaaaaa
      241 gcgttgctgt cccagcaaac acacctgtct acgctacagg tctgtaactg gttcatcaac
      301 gcccgccgca ggctcctccc tgacatgctg agaaaggatg gcaaagatcc aaatcagttc
      361 acaatttccc gccgtggggc caagatttct gaaacgagct ctgtggagtc cgtgatgggc
      421 atcaaaaact tcatgccagc tctagaggag accccatttc attcctgtac agctgggcca
      481 aacccaaccc tagggaggcc actgtctcct aagccgtcat ccccgggatc agttttggct
      541 cgtccatcag tgatctgcca taccactgtg actgcattga aagatgtccc tttctctctc
      601 tgccagtcgg tcggtgtggg acaaaacaca gatatacagc agatagcggc caaaaacttc
      661 acagacacct ctctcatgta cccagaggac acttgtaaat ctggaccaag tacgaataca
      721 cagagtggtc ttttcaacac tcctccccct actccaccgg acctcaacca ggacttcagt
      781 ggatttcagc ttctagtgga tgttgcactc aaacgggctg cagagatgga gcttcaggca
      841 aaacttacag ctaacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
      901 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
      961 tcgatttctt ggctttatat atcttgtgga aaggacgatc cctaattgaa catcctttgc
     1021 gt
//
