LOCUS       sequence-285540-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2999
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaactt ccaaaaggtg gaaaagatcg gagagggcac gtacggagtt
       61 gtgtacaaag ccagaaacaa gttgacggga gaggtggtgg cgcttaagaa aatccgcctg
      121 gacactgaga ctgagggtgt gcccagtact gccatccgag agatctctct gcttaaggag
      181 cttaaccatc ctaatattgt caagctgctg gatgtcattc acacagaaaa taaactctac
      241 ctggtttttg aatttctgca ccaagatctc aagaaattca tggatgcctc tgctctcact
      301 ggcattcctc ttcccctcat caagagctat ctgttccagc tgctccaggg cctagctttc
      361 tgccattctc atcgggtcct ccaccgagac cttaaacctc agaatctgct tattaacaca
      421 gagggggcca tcaagctagc agactttgga ctagccagag cttttggagt ccctgttcgt
      481 acttacaccc atgaggtggt gaccctgtgg taccgagctc ctgaaatcct cctgggctgc
      541 aaatattatt ccacagctgt ggacatctgg agcctgggct gcatctttgc tgagatggtg
      601 actcgccggg ccctattccc tggagattct gagattgacc agctcttccg gatctttcgg
      661 actctgggga ccccagatga ggtggtgtgg ccaggagtta cttctatgcc tgattacaag
      721 ccaagtttcc ccaagtgggc ccggcaagat tttagtaaag ttgtacctcc cctggatgaa
      781 gatggacgga gcttgttatc gcaaatgctg cactacgacc ctaacaagcg gatttcggcc
      841 aaggcagccc tggctcaccc tttcttccag gatgtgacca agccagtacc ccatcttcga
      901 ctctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      961 tgtggaaagg acgacttcgt gacaatacct ttcggaat
//
